Aggregation, Fusion, and Leakage of Liposomes Induced by Peptides

doi:10.1021/la501618f

CORC > 北京大学 > 化学与分子工程学院

	Aggregation, Fusion, and Leakage of Liposomes Induced by Peptides
	Xia, Yuqiong ; Sun, Jianbo ; Liang, Dehai
刊名	langmuir
	2014
关键词	PLASMA-MEMBRANE VESICLES SUPPORTED LIPID-BILAYERS BIOLOGICAL-MEMBRANES DOMAIN FORMATION FLUORESCENCE MICROSCOPY LATERAL ORGANIZATION MODEL MEMBRANES PROTEIN CURVATURE FLUCTUATIONS
DOI	10.1021/la501618f
英文摘要	Biological membranes are heterogeneous systems. Their functions are closely related to the lipid lateral segregation in the presence of membrane proteins. In this work, we designed two peptides, amphiphilic cationic peptides K3L8K3 and nonamphiphilic peptides K-20, and studied their interactions with binary liposomes in different phases (L-omega L-beta', and L-alpha/L-beta'). As mimics of membrane proteins, both K3L8K3 and K-20 can cause the liposomes to aggregate, fuse, or leak. These processes were closely related to the phases of liposomes. For the liposomes in L-alpha phase, heavy aggregation, fusion, and leakage were observed in the presence of either K-20 or K3L8K3. For the liposomes in L-beta' phase, neither K3L8K3 nor K-20 can induce fusion or leakage. For the liposomes in L-alpha/L-beta' phase, K3L8K3 caused the liposomes to aggregate, fuse, and leak, while K-20 only led to aggregation. The kinetics of aggregation, fusion, and leakage in each phase were recorded, and they were related to the lipid demixing in the presence of the peptide. Our work not only gained insight into the effect of the lipid demixing on the interactions between peptide and membrane, but also helped in developing drug delivery vehicles with liposomes as the platform.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000338488600011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary; SCI(E); EI; PubMed; 5; ARTICLE; dliang@pku.edu.cn; 25; 7334-7342; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188976]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xia, Yuqiong,Sun, Jianbo,Liang, Dehai. Aggregation, Fusion, and Leakage of Liposomes Induced by Peptides[J]. langmuir,2014.
APA	Xia, Yuqiong,Sun, Jianbo,&Liang, Dehai.(2014).Aggregation, Fusion, and Leakage of Liposomes Induced by Peptides.langmuir.
MLA	Xia, Yuqiong,et al."Aggregation, Fusion, and Leakage of Liposomes Induced by Peptides".langmuir (2014).