Dynamic behavior of small heat shock protein inhibition on amyloid   fibrillization of a small peptide (SSTSAA) from RNase A

doi:10.1016/j.bbrc.2011.11.010

CORC > 北京大学 > 化学与分子工程学院

	Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A
	Xi, Dong ; Dong, Xiao ; Deng, Wei ; Lai, Luhua
刊名	生物化学与生物物理学研究通讯
	2011
关键词	Amyloid fibril formation Inhibition Mj HSP16.5 Small heat shock protein FRET Electron microscopy ALPHA-B-CRYSTALLIN METHANOCOCCUS-JANNASCHII MOLECULAR CHAPERONE FIBRIL FORMATION TOXICITY AGGREGATION PREVENTS
DOI	10.1016/j.bbrc.2011.11.010
英文摘要	Small heat shock proteins, a class of molecular chaperones, are reported to inhibit amyloid fibril formation in vitro, while the mechanism of inhibition remains unknown. In the present study, we investigated the mechanism by which Mj HSP16.5 inhibits amyloid fibril formation of a small peptide (SSTSAA) from RNase A. A model peptide (dansyl-SSTSAA-W) was designed by introducing a pair of fluorescence resonance energy transfer (FRET) probes into the peptide, allowing for the monitoring of fibril formation by this experimental model. Mj HSP16.5 completely inhibited fibril formation of the model peptide at a molar ratio of 1:120. The dynamic process of fibril formation, revealed by FRET, circular dichroism, and electron microscopy, showed a lag phase of about 2 h followed by a fast growth period. The effect of Mj HSP16.5 on amyloid fibril formation was investigated by adding it into the incubation solution during different growth phases. Adding Mj HSP16.5 to the incubating peptide before or during the lag phase completely inhibited fibril formation. However, introducing Mj HSP16.5 after the lag phase only slowed down the fibril formation process by adhering to the already formed fibrils. These findings provide insight into the inhibitory roles of small heat shock proteins on amyloid fibril formation at the molecular level. (C) 2011 Elsevier Inc. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 4; ARTICLE; 1-2; 130-134; 416
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149936]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xi, Dong,Dong, Xiao,Deng, Wei,et al. Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A[J]. 生物化学与生物物理学研究通讯,2011.
APA	Xi, Dong,Dong, Xiao,Deng, Wei,&Lai, Luhua.(2011).Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A.生物化学与生物物理学研究通讯.
MLA	Xi, Dong,et al."Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A".生物化学与生物物理学研究通讯 (2011).