Highly Selective Detection of Single-Nucleotide Polymorphisms Using a   Quartz Crystal Microbalance Biosensor Based on the Toehold-Mediated   Strand Displacement Reaction

doi:10.1021/ac301064g

CORC > 北京大学 > 化学与分子工程学院

	Highly Selective Detection of Single-Nucleotide Polymorphisms Using a Quartz Crystal Microbalance Biosensor Based on the Toehold-Mediated Strand Displacement Reaction
	Wang, Dingzhong ; Tang, Wei ; Wu, Xiaojie ; Wang, Xinyi ; Chen, Gengjia ; Chen, Qiang ; Li, Na ; Liu, Feng
刊名	analytical chemistry
	2012
关键词	DNA-LIGASE REACTION SIGNAL AMPLIFICATION MOLECULAR BEACON PRIMER EXTENSION HYBRIDIZATION CLEAVAGE STRATEGY ENDONUCLEASE LIGATION TARGETS
DOI	10.1021/ac301064g
英文摘要	Toehold-mediated strand displacement reaction (SDR) is first introduced to develop a simple quartz crystal microbalance (QCM) biosensor without an enzyme or label at normal temperature for highly selective and sensitive detection of single-nucleotide polymorphism (SNP) in the p53 tumor suppressor gene. A hairpin capture probe with an external toehold is designed and immobilized on the gold electrode surface of QCM. A successive SDR is initiated by the target sequence hybridization with the toehold domain and ends with the unfolding of the capture probe. Finally, the open-loop capture probe hybridizes with the streptavidin-coupled reporter probe as an efficient mass amplifier to enhance the QCM signal. The proposed biosensor displays remarkable specificity to target the p53 gene fragment against single-base mutant sequences (e.g., the largest discrimination factor is 63 to C-C mismatch) and high sensitivity with the detection limit of 0.3 nM at 20 degrees C. As the crucial component of the fabricated biosensor for providing the high discrimination capability, the design rationale of the capture probe is further verified by fluorescence sensing and atomic force microscopy imaging. Additionally, a recovery of 84.1% is obtained when detecting the target sequence in spiked HeLa cells lysate, demonstrating the feasibility of employing this biosensor in detecting SNPs in biological samples.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000307695900015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); EI; PubMed; 58; ARTICLE; 16; 7008-7014; 84
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149861]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Dingzhong,Tang, Wei,Wu, Xiaojie,et al. Highly Selective Detection of Single-Nucleotide Polymorphisms Using a Quartz Crystal Microbalance Biosensor Based on the Toehold-Mediated Strand Displacement Reaction[J]. analytical chemistry,2012.
APA	Wang, Dingzhong.,Tang, Wei.,Wu, Xiaojie.,Wang, Xinyi.,Chen, Gengjia.,...&Liu, Feng.(2012).Highly Selective Detection of Single-Nucleotide Polymorphisms Using a Quartz Crystal Microbalance Biosensor Based on the Toehold-Mediated Strand Displacement Reaction.analytical chemistry.
MLA	Wang, Dingzhong,et al."Highly Selective Detection of Single-Nucleotide Polymorphisms Using a Quartz Crystal Microbalance Biosensor Based on the Toehold-Mediated Strand Displacement Reaction".analytical chemistry (2012).