| 用铀试剂Ⅰ共振光散射法测定蛋白质的研究; Study on determination of protein with Arsenazo I using resonance light scattering technique | |
| 李树伟 ; 李娜 ; 赵凤林 ; 李克安 | |
| 2002 | |
| 关键词 | 共振光散射 蛋白质 铀试剂Ⅰ resonance light scattering protein Arsenazo I |
| 英文摘要 | 本文提出一种新的光散射探针染料———铀试剂Ⅰ (ArsenazoⅠ ) ,在pH 3 2 9的条件下 ,ArsenazoⅠ本身只有极弱的光散射 ,它与蛋白质的结合物有强烈的共振光散射作用。在λ =4 0 0nm处 ,光散射有最大强度 ,光散射强度与蛋白质的浓度成正比。据此建立了一种测定蛋白质的新方法。该方法简便、快速、灵敏度高 ,对HSA的检测限达到 6 0ng·mL-1 ,线性范围达到 0～ 18μg·mL-1 ,用于人体血清样品的分析并同考马斯亮蓝法比较 ,取得了令人满意的结果。本文也研究了牛血清白蛋白 (BSA)、γ球蛋白、鸡蛋白蛋白、溶菌酶、胃蛋白酶与染料ArsonazoⅠ之间的作用 ...; A new RLS (Resonance light scattering) probe is presented in this paper. In the Britton-Robinson buffer at, pH 3.29, Arsenazo I combines with proteins by intermolecular forces, resulted in an enhanced Rayleigh light scattering. The RLS intensity reaches maximum at 400 rim and is proportional to the concentration of protein. A novel method for the determination of micro-amount of protein is developed. The assay is simple, rapid and sensitive with a detection limit at 60 ng(.)mL(-1) and a linear range up to 18 mug(.)mL(-1). This method is applied to the determination of human serum protein, and the results, compared to Bradford method, is satisfactory. The interaction of Arsenazo I with BSA, gamma-G, oval albumin, lysozyme, pepsin are investigated and the mechanism of RLS is also discussed.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000177804700026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); EI; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 5; 04; 619-622; 22 |
| 语种 | 中文 |
| 出处 | 万方 ; 知网 ; SCI ; PubMed |
| 出版者 | 光谱学与光谱分析 |
| 内容类型 | 其他 |
| 源URL | [http://hdl.handle.net/20.500.11897/77955]  |
| 专题 | 化学与分子工程学院 |
| 推荐引用方式 GB/T 7714 | 李树伟,李娜,赵凤林,等. 用铀试剂Ⅰ共振光散射法测定蛋白质的研究, Study on determination of protein with Arsenazo I using resonance light scattering technique. 2002-01-01. |
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