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	氧氟沙星的荧光光谱与质子化作用研究; Fluorescence spectra and protonation of ofloxacin
	刘翠格 ; 徐怡庄 ; 魏永巨 ; 齐剑 ; 许振华 ; 叶放 ; 吴瑾光
	2005
关键词	氧氟沙星 喹诺酮 荧光光谱 紫外光谱 质子化 Ofloxacin quinolone fluorescence spectrum ultraviolet spectrum protonation
英文摘要	研究了氧氟沙星(Ofloxacin ,OFL)在不同pH条件下的荧光光谱、紫外光谱和质子化作用。在强酸性溶液中,OFL分子可以结合两个质子而以三元酸H3 L2 + 的形式存在,最大荧光发射波长(λmax)为5 0 5nm。随着pH值升高,OFL的荧光光谱发生变化,在激发光谱中35 2nm形成一等荧光点,同时在紫外吸收光谱中出现等色点,这一光谱特征表明H3 L2 + 逐渐失去4位C羰基氧结合的质子。在pH 2 . 5～4范围内,OFL以H2 L+ 形式存在,λmax为4 99nm。当pH >4时,随pH值升高,位于4 99nm的荧光发射峰逐渐蓝移至4 5 5nm ,在4 84nm形成一等荧光发射...; Fluorescence spectra, ultraviolet spectra and protonation of Ofloxacin (OFL) at different pH values have been studied. In strong acidic solutions, OFL molecule might accept two protons to exist as ternary acid H3L2+ with a maximum emission wavelength (lambdamax) at 505 nm. Along with the increase in pH value, fluorescence spectrum of OFL changed, an iso-fluorescence point at 352 nm was found in the fluorescence excitation spectra, and at the same time, isosbestic points were found in its UV absorption spectra. This spectral feature reveals that H3L2+ gradually lost hydrogen ion combined on C-4 keto oxygen. In the range of pH 2.5 to pH 4, OFL exists as H2L+ with lambdamax at 499 nm. When pH>4, the fluorescence emission peak at 499 nm gradually blue-shifted to 455 nm as pH was increasing, and an iso-fluorescence emission point was formed at 484 nm, indicating the dissociation of hydrogen ion of carboxylic acid at C-3. At about pH 7.0, OFL exists in the dipole ion form HL with lambdamax at 455 nm, which is the strongest fluorescence form. When pH>8, lambdamax red-shift from 455 to 475 nm as pH increases, meanwhile, fluorescence intensity decreases, indicating that HL lost hydrogen ion combined on N-4 of piperazinyl group. When pH>10, OFL exists in anion ion form L-, and fluorescence intensity decreases with pH increasing, but lambdamax remains essentially constant, indicating that medium environment influences the fluorescence property of OFL.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000228943600029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); EI; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 13; 04; 584-587; 25
语种	中文
出处	PubMed ; SCI ; 万方 ; 知网
出版者	光谱学与光谱分析
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/77924]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	刘翠格,徐怡庄,魏永巨,等. 氧氟沙星的荧光光谱与质子化作用研究, Fluorescence spectra and protonation of ofloxacin. 2005-01-01.

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