| 光谱法研究3H-吲哚季铵盐探针分子与牛血清底的相互作用; Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence | |
| 徐同宽 ; 沈兴海 ; 高宏成 | |
| 2005 | |
| 关键词 | 取代3H-吲哚季铵盐 合成 牛血清蛋白 荧光光谱 substituted 3H-indole quaternary ammonium molecule synthesis BSA fluorescence |
| 英文摘要 | 合成了一种新型的荧光探针分子2-(对-十二烷基氨基)苯基-3, 3-二甲基-5-乙酯基-3H-吲哚基-甲基-二-十六烷基碘化铵, 并运用荧光光谱探讨它与牛血清蛋白的作用. 结果表明, 探针分子与牛血清蛋白(BSA)的结合作用可使3H-吲哚的荧光强度增强, 而对蛋白质却具有猝灭性质, 其结合常数和结合位点数分别为Ka=1.995×105 dm3*mol-1和n=1.12.; A substituted 3H-indole quaternary ammonium molecule was designed and synthesized using hexamethylphosphoramide (HMPA) as a solvent. The products were purified and characterized by IR, H-1-NMR, MS and elemental analysis. The binding reaction of this compound with bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. Their binding constant is K-a = 1.995 x 10(5) dm(3) center dot mol(-1) and the binding site number is n = 1.12. It is confirmed that the combination is a single static quenching process.; 国家自然科学基金; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000230381500015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); EI; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 4; 6; 857-860; 25 |
| 语种 | 中文 |
| 出处 | PubMed ; SCI ; 万方 ; http://d.g.wanfangdata.com.cn/Periodical_gpxygpfx200506015.aspx |
| 出版者 | 光谱学与光谱分析 |
| 内容类型 | 其他 |
| 源URL | [http://hdl.handle.net/20.500.11897/77923]  |
| 专题 | 化学与分子工程学院 |
| 推荐引用方式 GB/T 7714 | 徐同宽,沈兴海,高宏成. 光谱法研究3H-吲哚季铵盐探针分子与牛血清底的相互作用, Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence. 2005-01-01. |
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