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	微电纯化装置用于重组人促红细胞生成素的质谱分析前样品快速除盐; Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection
	庞楠楠 ; 周宇 ; 白玉 ; 廖杰 ; 刘虎威
	2011
关键词	液相色谱 质谱 微电纯化装置 超滤 重组人促红细胞生成素 Liquid chromatography Mass spectrometry Microelectro purification device Ultra-filtration Recombinant human erythropoietin
英文摘要	利用实验室构建的微电纯化装置(Microelectro purification device,MEPD),采用电喷雾质谱正离子模式检测,在2 min之内实现重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)的在线脱盐,同时和传统的C18色谱柱(150 mm×4.6 mm,5μm)梯度洗脱以及超滤管除盐结果进行比较。利用MEPD除盐后,rhEPO特征离子信噪比增加,检测灵敏度明显提高,操作时间大大缩短。本方法简单、快速,可作为质谱分析rhEPO样品的前处理方法。; Microelectro purification device (MEPD) constructed by our laboratory could obtain online desalting of recombinant human erythropoietin (rhEPO) in 2 min with high efficiency. Electrospray mass spectrometry with positive ion mode was used for the detection. Compared with traditional methods, such as gradient elution by C(18)-reversed phase liquid chromatography (150 mm X4. 6 mm, 5 mu m) as well as ultra-filtration, MEPD could further enhance the signal to noise ratio of rhEPO and the detection sensitivity. It could also shorten the analysis time dramatically. This simple and rapid method could be a promising pretreatment candidate for the rhEPO analysis of mass spectrometry.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000290240800015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中国科学引文数据库(CSCD); 0; 02; 231-234; 39
语种	中文
出处	知网 ; 万方 ; SCI
出版者	分析化学
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/46014]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	庞楠楠,周宇,白玉,等. 微电纯化装置用于重组人促红细胞生成素的质谱分析前样品快速除盐, Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection. 2011-01-01.

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