QQ客服 官方微博 反馈留言

	An allosteric model of the inositol trisphosphate receptor with nonequilibrium binding
	Jia, Chen ; Jiang, Daquan ; Qian, Minping
	2014
关键词	adaptation overshoot nonequilibrium Monod-Wyman-Changeux model Xenopus oocyte patch-clamp SINGLE-CHANNEL KINETICS CA2+ RELEASE CHANNELS 1,4,5-TRISPHOSPHATE RECEPTOR IP3 RECEPTORS PARTIAL INACTIVATION RAPID ACTIVATION STEADY-STATE CALCIUM ADAPTATION PHOSPHORYLATION
英文摘要	The inositol trisphosphate receptor (IPR) is a crucial ion channel that regulates the Ca2+ influx from the endoplasmic reticulum (ER) to the cytoplasm. A thorough study of the IPR channel contributes to a better understanding of calcium oscillations and waves. It has long been observed that the IPR channel is a typical biological system which performs adaptation. However, recent advances on the physical essence of adaptation show that adaptation systems with a negative feedback mechanism, such as the IPR channel, must break detailed balance and always operate out of equilibrium with energy dissipation. Almost all previous IPR models are equilibrium models assuming detailed balance and thus violate the dissipative nature of adaptation. In this article, we constructed a nonequilibrium allosteric model of single IPR channels based on the patch-clamp experimental data obtained from the IPR in the outer membranes of isolated nuclei of the Xenopus oocyte. It turns out that our model reproduces the patch-clamp experimental data reasonably well and produces both the correct steady-state and dynamic properties of the channel. Particularly, our model successfully describes the complicated bimodal [Ca2+] dependence of the mean open duration at high [IP3], a steady-state behavior which fails to be correctly described in previous IPR models. Finally, we used the patch-clamp experimental data to validate that the IPR channel indeed breaks detailed balance and thus is a nonequilibrium system which consumes energy.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000343670600018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biophysics; SCI(E); PubMed; 3; ARTICLE; jiangdq@math.pku.edu.cn; 5; 056001; 11
语种	英语
出处	PubMed ; SCI
出版者	physical biology
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/157227]
专题	数学科学学院
推荐引用方式 GB/T 7714	Jia, Chen,Jiang, Daquan,Qian, Minping. An allosteric model of the inositol trisphosphate receptor with nonequilibrium binding. 2014-01-01.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。