Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition

doi:10.1166/jbt.2018.1877

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	Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition
	Li, LM ; Liu, XQ ; Guo, P ; Ren, YB ; Yang, K 1
刊名	JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
	2018-09-01
卷号	8 期号:9 页码:1320-1326
关键词	In-Stent Restenosis Nickel-Free Stainless Steel Migration Epithelial-Mesenchymal Transition
ISSN号	2157-9083
DOI	10.1166/jbt.2018.1877
英文摘要	To understand the impact of high-nitrogen nickel-free austenitic stainless steel (HNNFSS) on the migration and epithelial-mesenchymal transition (EMT) of human umbilical vein endothelial cells (HUVECs) and the involved mechanisms, we conducted a comparative study of cellular and molecular responses of HUVECs to HNNFSS and 317L (austenitic 317L stainless steel). Cell Counting Kit-8 assay was used to analyze cell viability. The migration capabilities of HUVECs were analyzed using the wound healing and Transwell assays. Fluorescence staining was performed to observe the cell cytoskeleton. Quantitative real-time polymerase chain reaction and Western blot were conducted to analyze the gene expression and protein levels associated with migration and EMT-related markers. Our results showed that HNNFSS promoted HUVEC migration and increased matrix metalloproteinase (MMP)-2 and MMP-9 expression and activities. In addition, HNNFSS treatment induced cytoskeletal remodeling and increased the expression of related genes. The treatment also stimulated EMT as characterized by the decrease in E-cadherin and the increase in vimentin. Our results suggest that compared with 317L, HNNFSS enhances the migration capabilities of HUVECs and promotes EMT. The findings of this study provide valuable information fot developing new biomedical materials for stent implantation.
学科主题	Cell & Tissue Engineering
语种	英语
WOS记录号	WOS:000448343200013
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/79974]
专题	金属研究所_中国科学院金属研究所
作者单位	1.Northeastern Univ, Coll Life & Hlth Sci, Inst Biotechnol, Shenyang 110819, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Li, LM,Liu, XQ,Guo, P,et al. Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition[J]. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,2018,8(9):1320-1326.
APA	Li, LM,Liu, XQ,Guo, P,Ren, YB,&Yang, K.(2018).Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition.JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,8(9),1320-1326.
MLA	Li, LM,et al."Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition".JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING 8.9(2018):1320-1326.