Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation

doi:10.1002/jbm.b.34005

CORC > 金属研究所 > 中国科学院金属研究所

	Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation
	Zhao, J; Ren, L; Liu, MX; Xi, T; Zhang, BC; Yang, K
刊名	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
	2018-07-01
卷号	106 期号:5 页码:2019-2028
关键词	Cu bearing stainless steel fibrosis CuZnSOD TGF-beta 1 Smad3 COL1 COL3
ISSN号	1552-4973
DOI	10.1002/jbm.b.34005
英文摘要	Recurrent stenosis is the main reason inducing the failure of urethral stricture treatment. Our previous study has found that the 316L type Cu bearing stainless steel (316L-Cu SS) showed antimicrobial activity and anti-encrustation performance when it was used for relieving urethral obstructer. However, whether it can reduce the occurrence of fibrosis or not, we need further investigation to compare the cellular and molecular responses of human urethral scar fibroblast cells (USFCs) on 316L-Cu SS and medical grade 316L stainless (316L SS, as a control). [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)- 2H-tetrazolium (MTS) and Transwell were used to assess the cellular responses, which confirmed that 316L-Cu SS could inhibit proliferation and migration of USFCs. Molecular expressions of fibrosis were evaluated by western blot, real-time quantitative polymerase chain reaction (qPCR), and Cu/Zn superoxide dismutase (CuZnSOD) measurement. The results indicated that up-regulating of CuZnSOD attenuated the transforming growth factor-1 expression and phosphorylation of Smad3 after exposure to 316L-Cu SS. Besides, the content of collagen type I (COL1) and collagen type III (COL3) secreting into the culture medium measured by enzyme-linked immunosorbent assay were in accord with the results of messenger ribonucleic acids. Both of them exhibited lower levels of COL1/COL3 exposure to 316L-Cu SS, demonstrating the inhibitory performance of 316L-Cu SS against fibrosis. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2019-2028, 2018.
学科主题	Engineering, Biomedical ; Materials Science, bioMaterials
语种	英语
WOS记录号	WOS:000435443500038
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/80124]
专题	金属研究所_中国科学院金属研究所
作者单位	Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhao, J,Ren, L,Liu, MX,et al. Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2018,106(5):2019-2028.
APA	Zhao, J,Ren, L,Liu, MX,Xi, T,Zhang, BC,&Yang, K.(2018).Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,106(5),2019-2028.
MLA	Zhao, J,et al."Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 106.5(2018):2019-2028.