Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach

doi:10.1021/acs.analchem.5b04102

	Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach
	Liu L(刘蕾); Li YR(李玉茹); Li T(李婷); Xie JN(谢佳妮); Chen CF(陈超飞); Li, YR; Liu, L; Wu HC(吴海臣); Liu QS(刘全胜); Zhang, SW
刊名	ANALYTICAL CHEMISTRY
	2016
卷号	88 期号:2 页码:1073-1077
ISSN号	0003-2700
DOI	10.1021/acs.analchem.5b04102
文献子类	Article
英文摘要	We have developed a nanopore sensing approach for the selective detection of 8-oxo-2'-deoxyguanosine (8-oxoG) in single-stranded DNA. First, 1,12-dodecanediamine is coupled with 8-oxoG-containing DNA molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. After incubation with cucurbit[7]uril, the host-guest complex-modified DNA hybrid is translocated through an alpha-hemolysin nanopore. Highly characteristic events can be recorded and used to quantify the 8-oxoG-DNA content in a DNA mixture. Compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxoG site in single-stranded DNA at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples.
电子版国际标准刊号	1520-6882
语种	英语
WOS记录号	WOS:000368562300002
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/287079]
专题	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Liu L,Li YR,Li T,et al. Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach[J]. ANALYTICAL CHEMISTRY,2016,88(2):1073-1077.
APA	刘蕾.,李玉茹.,李婷.,谢佳妮.,陈超飞.,...&Wu, HC.(2016).Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach.ANALYTICAL CHEMISTRY,88(2),1073-1077.
MLA	刘蕾,et al."Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach".ANALYTICAL CHEMISTRY 88.2(2016):1073-1077.