Investigating oxidation state-induced toxicity of PEGylated graphene oxide in ocular tissue using gene expression profiles

doi:10.1080/17435390.2018.1480813

	Investigating oxidation state-induced toxicity of PEGylated graphene oxide in ocular tissue using gene expression profiles
	Yin, Z. Q.; Wu, W.; Yan L(晏亮); Yan, L.; Chen, S.; Li, Q.; Gu, Z.; Xu, H.; Gu ZJ(谷战军)
刊名	NANOTOXICOLOGY
	2018
卷号	12 期号:8 页码:819-835
关键词	Cytotoxicity graphene oxide physico-chemical properties gene expression profiles oxidative stress
ISSN号	1743-5390
DOI	10.1080/17435390.2018.1480813
文献子类	Article
英文摘要	Graphene and its derivatives are widely used for a variety of industrial, biomedical, and environmental applications. However, the potential harm caused by exposure of the eyes to graphene-based nanomaterials is scarce. Given the potential for these materials to be used in multiple applications, there is a pressing need to evaluate their ocular toxicity, and understand the relationships between their physico-chemical properties and the resulting toxicity. In this study, the toxicity of PEGylated graphene oxide (PEG-GO) with differing oxidation levels and/or surface charges (positive, negative and neutral charge) was evaluated using two in-vitro models of the eye: primary human corneal epithelial cells and human retinal capillary endothelial cells. The results showed that oxidation level, but not surface charge, had a pivotal effect on the toxicity of graphene-based nanomaterials. Typically, PEG-GO sample with a higher oxidation level caused more serious cytotoxicity than those with a lower oxidation level. Furthermore, by analysis of global gene expression profiles, we found that the foremost cellular response to PEG-GO sample with a high oxidation level was the oxidative stress response. Next, via exploring the underlying molecular mechanism of oxidative stress-induced cytotoxicity, we showed that PEG-GO sample with a high degree of oxidation induced reactive oxygen species (ROS) via NDUFB9-mediated biological pathway. This work has significant implications for design of safe graphene-based nanomaterials for biomedical applications.
电子版国际标准刊号	1743-5404
WOS关键词	WALLED CARBON NANOTUBES ; IN-VITRO ; NANOPARTICLES ; CYTOTOXICITY ; BIOCOMPATIBILITY ; POLYETHYLENIMINE ; GENOTOXICITY ; NANOCARRIER ; MEMBRANES ; EXPOSURE
WOS研究方向	Science & Technology - Other Topics ; Toxicology
语种	英语
WOS记录号	WOS:000452281100002
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286894]
专题	高能物理研究所_多学科研究中心
通讯作者	Gu ZJ(谷战军)
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Yin, Z. Q.,Wu, W.,Yan L,et al. Investigating oxidation state-induced toxicity of PEGylated graphene oxide in ocular tissue using gene expression profiles[J]. NANOTOXICOLOGY,2018,12(8):819-835.
APA	Yin, Z. Q..,Wu, W..,晏亮.,Yan, L..,Chen, S..,...&谷战军.(2018).Investigating oxidation state-induced toxicity of PEGylated graphene oxide in ocular tissue using gene expression profiles.NANOTOXICOLOGY,12(8),819-835.
MLA	Yin, Z. Q.,et al."Investigating oxidation state-induced toxicity of PEGylated graphene oxide in ocular tissue using gene expression profiles".NANOTOXICOLOGY 12.8(2018):819-835.