Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer

doi:10.1021/acsami.8b10252

	Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer
	Yang, Dayong; Cheng LX(程林秀); Cheng, Linxiu; Zhang, Zhikun; Zuo, Duo; Zhu, Wenfeng; Zhang, Jie; Zeng, Qingdao; Li, Min; Zhao, Yuliang
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018
卷号	10 期号:41 页码:34869-34877
关键词	frequency-shift biosensing SERS ultrasensitive detection branched DNA liver cancer biomarker
ISSN号	1944-8244
DOI	10.1021/acsami.8b10252
文献子类	Article
英文摘要	We provided an ultrasensitive sensing strategy for microRNA detection by first employing branched DNA. With the aid of microcontact printing, we realized the multiplex sensing of different kinds of liver cancer biomarkers: microRNA and protein simultaneously. Delicately designed branched DNA included multiple complementary sticky ends as probe to microRNA capture and the double-stranded rigid branched core to increase the active sticky-ends distance and expose more DNA probes for sensitivity. The branched DNA enables 2 orders of magnitude increase in sensitivity for microRNA detection over single-stranded DNA. The limit of detection reaches as low as 10 attomolar (S/N = 3) for miR-223 and 10(-12) M for alpha-fetoprotein. In addition, this system shows high selectivity and appropriate reproducibility (the relative standard deviation is less than 20%) in physiological media. Serum samples are tested and the results of alpha-fetoprotein are in good agreement with the current gold-standard method, electrochemiluminescence immunoassay analyzer. The results suggest the reliability of this approach in physiological media and show high potential in the sensing of low abundant microRNA in serum, especially for early diagnosis of primary liver cancers.
WOS关键词	ENHANCED RAMAN-SCATTERING ; HEPATOCELLULAR-CARCINOMA ; NANOPARTICLE PROBES ; STRANDED-DNA ; IMMUNOASSAY ; BIOMARKERS ; CELLS ; NANOSTRUCTURES ; SENSOR ; QUANTIFICATION
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
WOS记录号	WOS:000447954600008
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286618]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Yang, Dayong,Cheng LX,Cheng, Linxiu,et al. Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(41):34869-34877.
APA	Yang, Dayong.,程林秀.,Cheng, Linxiu.,Zhang, Zhikun.,Zuo, Duo.,...&赵宇亮.(2018).Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer.ACS APPLIED MATERIALS & INTERFACES,10(41),34869-34877.
MLA	Yang, Dayong,et al."Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer".ACS APPLIED MATERIALS & INTERFACES 10.41(2018):34869-34877.