Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology

doi:10.1021/acs.nanolett.8b01805

	Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology
	Xie, GC; Gao, SJ; Shi, HG; Zhou, MX; Zhou MX(周梦雪); Tian, Y; Chen J(陈俊); Niu, ZW; Chen, J; Wu, M
刊名	NANO LETTERS
	2018
卷号	18 期号:9 页码:5453-5460
关键词	Tobacco mosaic virus cell-penetrating peptide gene silence endosomal escape viral vector
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.8b01805
文献子类	Article
英文摘要	Inspired by the high gene transfer efficiency of viral vectors and to avoid side effects, we present here a 1D rod-like gene-silencing vector based on a plant virus. By decorating the transacting activator of transduction (TAT) peptide on the exterior surface, the TAT-modified tobacco mosaic virus (TMV) achieves a tunable isoelectric point (from similar to 3.5 to similar to 9.6) depending on the TAT dose. In addition to enhanced cell internalization, this plant virus based vector (TMV-TAT) acquired endo/lysosomal escape capacity without inducing lysosomal damage, resulting in both high efficiency and low cytotoxicity. By loading silencer green fluorescent protein (GFP) siRNA onto the TMV-TAT vector (siRNA@TMV-TAT) and interfering with GFP-expressing mouse epidermal stem cells (ESCs/GFP) in vitro, the proportion of GFP-positive cells could be knocked down to levels even lower than 15% at a concentration of similar to 100% cell viability. Moreover, by interfering with GFP-expressing highly metastatic hepatocellular carcinoma (MHCC97-H/GFP) tumors in vivo, treatment with siRNA@TMV-TAT complexes for 10 days achieved a GFP-negative rate as high as 80.8%. This work combines the high efficiency of viral vectors and the safety of nonviral vectors and may provide a promising strategy for gene-silencing technology.
电子版国际标准刊号	1530-6992
WOS关键词	TOBACCO-MOSAIC-VIRUS ; MULTIWALL CARBON NANOTUBES ; RNA INTERFERENCE ; DRUG-DELIVERY ; NANOPARTICLES ; VECTOR ; FLUORESCENCE ; THERAPY ; PROTEINS ; CARRIERS
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000444793500016
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286333]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Xie, GC,Gao, SJ,Shi, HG,et al. Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology[J]. NANO LETTERS,2018,18(9):5453-5460.
APA	Xie, GC.,Gao, SJ.,Shi, HG.,Zhou, MX.,周梦雪.,...&Zhu, M.(2018).Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology.NANO LETTERS,18(9),5453-5460.
MLA	Xie, GC,et al."Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology".NANO LETTERS 18.9(2018):5453-5460.