Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing

doi:10.1002/anie.201803324

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	Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing
	Zhang, SW; Liu, L; Guo BY(郭秉元); Zhao YL(赵宇亮); Li T(李婷); 刘蕾(多); Wu, HC; Zhao, YL; Guo, BY; Song, P
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2018
卷号	57 期号:37 页码:11882-11887
关键词	antigens barcode DNA biomarkers multiplexed detection nanopore sensing
ISSN号	1433-7851
DOI	10.1002/anie.201803324
文献子类	Article
英文摘要	Protein biomarkers in blood have been widely used in the early diagnosis of disease. However, simultaneous detection of many biomarkers in a single sample remains challenging. Herein, we show that the combination of a sandwich assay and DNA-assisted nanopore sensing could unambiguously identify and quantify several antigens in a mixture. We use five barcode DNAs to label different gold nanoparticles that can selectively bind specific antigens. After the completion of the sandwich assay, barcode DNAs are released and subject to nanopore translocation tests. The distinct current signatures generated by each barcode DNA allow simultaneous quantification of biomarkers at picomolar level in clinical samples. This approach would be very useful for accurate and multiplexed quantification of cancer-associated biomarkers within a very small sample volume, which is critical for non-invasive early diagnosis of cancer.
电子版国际标准刊号	1521-3773
WOS关键词	ALPHA-HEMOLYSIN NANOPORE ; SOLID-STATE ; REAL-TIME ; STOCHASTIC DETECTION ; BIOLOGICAL NANOPORE ; NANOPARTICLE PROBES ; PROTEIN NANOPORE ; LABEL-FREE ; SINGLE ; DISCRIMINATION
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000443675700003
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286283]
专题	高能物理研究所_核技术应用研究中心高能物理研究所_粒子天体物理中心高能物理研究所_多学科研究中心
通讯作者	刘蕾(多)
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Zhang, SW,Liu, L,Guo BY,et al. Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018,57(37):11882-11887.
APA	Zhang, SW.,Liu, L.,郭秉元.,赵宇亮.,李婷.,...&Li, T.(2018).Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,57(37),11882-11887.
MLA	Zhang, SW,et al."Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.37(2018):11882-11887.