Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella) | |
Zhu, Denghui1,2; Huang, Rong1; Yang, Cheng1,2; Fu, Peipei1,4,5; Chen, Liangming1,2; Jiang, Yinjun1,2; He, Libo1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1 | |
刊名 | FISH & SHELLFISH IMMUNOLOGY |
2019-09-01 | |
卷号 | 92期号:1页码:570-582 |
关键词 | Grass carp Recombinant protein Peroxiredoxin 2 (Prx2) Subcellular localization Gene expression Antioxidant activity Immune response |
ISSN号 | 1050-4648 |
DOI | 10.1016/j.fsi.2019.06.022 |
英文摘要 | Peroxiredoxin (Prx), also named thioredoxin peroxidase (TPx), is a selenium independent antioxidant enzyme that can protect organisms from oxidative damage caused by reactive oxygen species (ROS) and is important for immune responses. In this study, the molecular cloning and characterization of a Prx2 homologue (CiPrx2) were described from grass carp (Ctenoplicuyngodon idella). The full-length cDNA of CiPrx2 was 1163 by containing 5'-untranslated region (UTR) of 52 bp, a 3'-UTR of 517 by with the putative polyadenylation consensus signal (AATAAA), an open reading frame (ORF) of 594 by encoding polypeptides of 197 amino acids with a predicted molecular mass of 21.84 kDa and theoretical isoelectric point of 5.93. The analysis results of multiple sequence alignment and phylogenetic tree confirmed that CiPrx2 belong to the typical 2-Cys Prx subfamily. The CiPrx2 mRNA was ubiquitously expressed in all tested tissues. The temporal expression of CiPrx2 were differentially induced infected with grass carp reovirus (GCRV), polyinosinic:polycytidylic acid (poly I:C) and lipopolysaccharide (LPS) in liver and spleen. Subcellular localization of CiPrx2-GFP fusion proteins were only distributed in the cytoplasm. The purified recombinant CiPrx2 possessed an apparent antioxidant activity and could protect DNA against oxidative damage. Finally, CiPrx2 proteins could obviously inhibit H2O2 and heavy metal toxicity. However, further researches are needed to better understand the regulation of CiPrx2 under oxidative stresses. |
资助项目 | National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31572614] |
WOS关键词 | 2-CYS PEROXIREDOXIN ; OXIDATIVE STRESS ; REACTIVE OXYGEN ; IMMUNE-RESPONSE ; GENE FAMILY ; FUNCTIONAL-CHARACTERIZATION ; ANTIOXIDANT DEFENSE ; EXPRESSION PROFILES ; REDOX REGULATION ; MIIUY CROAKER |
WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
语种 | 英语 |
出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000483634300062 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/33317] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Wang, Yaping |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Denghui,Huang, Rong,Yang, Cheng,et al. Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,92(1):570-582. |
APA | Zhu, Denghui.,Huang, Rong.,Yang, Cheng.,Fu, Peipei.,Chen, Liangming.,...&Wang, Yaping.(2019).Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,92(1),570-582. |
MLA | Zhu, Denghui,et al."Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 92.1(2019):570-582. |
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