Molecular cloning of the MARCH family in grass carp (Ctenopharyngodon idellus) and their response to grass carp reovirus challenge | |
Ou, Mi1,2; Huang, Rong1; Xiong, Lv1,2; Luo, Lifei1,2; Chen, Geng1,2; Liao, Lanjie1; Li, Yongming1; He, Libo1; Zhu, Zuoyan1; Wang, Yaping1 | |
刊名 | FISH & SHELLFISH IMMUNOLOGY |
2017-04-01 | |
卷号 | 63期号:1页码:480-490 |
关键词 | Grass carp GCRV MARCH qRT-PCR Immune-related genes |
ISSN号 | 1050-4648 |
DOI | 10.1016/j.fsi.2017.02.030 |
英文摘要 | Grass carp (Ctenopharyngodon idellus) is an economical aquaculture species in China, and the Grass Carp Reovirus (GCRV) that causes hemorrhagic disease seriously affects the grass carp cultivation industry. Substantial evidence indicates that there is an association between the membrane-associated RING-CH family of E3 ligase (MARCH) family and immune defense in mammals, while functional studies on non mammalian MARCH proteins are limited. In order to know the characteristics of the MARCH genes in C. idellus, eight MARCH genes (MARCHI, 2, 5, 6, 7, 8, 9 and 11) were cloned and the open reading frames (ORF) were identified in grass carp. All MARCH proteins in grass carp contained an RING-CH domain, which is characteristic of the MARCH protein. The phylogenetic analysis revealed that different MARCH proteins gathered into their separate clusters. All eight members of the MARCH gene family were detected in all tissues sampled, but the relative expression level differed. In addition, the mRNA expression of all the MARCHs was regulated at different levels in the immune organs after a GCRV challenge, and they responded robustly in both the intestine and liver. The mRNA expression of MARCH8, MHC 11, TfR, IL1RAP, EGR1, and DUSPI in the intestine after GCRV infection was analyzed, and the results showed that MARCH8 could negatively regulate TfR, IL1RAP, EGR1, and DUSPI, which signaled via the MAPK or NF-kappa B-activation pathways that play vital roles in immunity. Our findings identified a novel gene family in C. idellus and provided novel evidence that MARCH genes are inducible and involved in the immune response. Moreover, MARCH8 might function to negatively regulate immune receptors in C idellus. Therefore, the MARCH might play a vital role in regulating the immune response of C idellus. (C) 2017 Elsevier Ltd. All rights reserved. |
资助项目 | National Natural Science Foundation of China[31572614] ; National Natural Science Foundation of China[31130055] ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science[XDA08030203] |
WOS关键词 | NF-KAPPA-B ; UBIQUITIN E3 LIGASES ; DOWN-REGULATION ; CLASS-I ; ANTIGEN PRESENTATION ; RAINBOW-TROUT ; INFECTION ; DEGRADATION ; EXPRESSION ; GENOME |
WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
语种 | 英语 |
出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000398870700052 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/32765] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Wang, Yaping |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Ou, Mi,Huang, Rong,Xiong, Lv,et al. Molecular cloning of the MARCH family in grass carp (Ctenopharyngodon idellus) and their response to grass carp reovirus challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2017,63(1):480-490. |
APA | Ou, Mi.,Huang, Rong.,Xiong, Lv.,Luo, Lifei.,Chen, Geng.,...&Wang, Yaping.(2017).Molecular cloning of the MARCH family in grass carp (Ctenopharyngodon idellus) and their response to grass carp reovirus challenge.FISH & SHELLFISH IMMUNOLOGY,63(1),480-490. |
MLA | Ou, Mi,et al."Molecular cloning of the MARCH family in grass carp (Ctenopharyngodon idellus) and their response to grass carp reovirus challenge".FISH & SHELLFISH IMMUNOLOGY 63.1(2017):480-490. |
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