Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection | |
He, Libo1; Zhang, Aidi1; Xiong, Lv1,2; Li, Yongming1; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1 | |
刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
2017-09-01 | |
卷号 | 18期号:9页码:16 |
关键词 | grass carp grass carp reovirus circRNA differential expression paternal gene binding miRNA blood coagulation hemorrhagic symptoms mRNA-miRNA-circRNA network |
ISSN号 | 1422-0067 |
DOI | 10.3390/ijms18091977 |
英文摘要 | Grass carp hemorrhagic disease, caused by the grass carp reovirus (GCRV), is a major disease that hampers the development of grass carp aquaculture in China. The mechanism underlying GCRV infection is still largely unknown. Circular RNAs (circRNAs) are important regulators involved in various biological processes. In the present study, grass carp were infected with GCRV, and spleen samples were collected at 0 (control), 1, 3, 5, and 7 days post-infection (dpi). Samples were used to construct and sequence circRNA libraries, and a total of 5052 circRNAs were identified before and after GCRV infection, of which 41 exhibited differential expression compared with controls. Many parental genes of the differentially expressed circRNAs are involved in metal ion binding, protein ubiquitination, enzyme activity, and nucleotide binding. Moreover, 72 binding miRNAs were predicted from the differentially expressed circRNAs, of which eight targeted genes were predicted to be involved in immune responses, blood coagulation, hemostasis, and complement and coagulation cascades. Upregulation of these genes may lead to endothelial and blood cell damage and hemorrhagic symptoms. Our results indicate that an mRNA-miRNA-circRNA network may be present in grass carp infected with GCRV, providing new insight into the mechanism underlying grass carp reovirus infection. |
资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030203] ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2017FB12] ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences[Y65E021301] |
WOS关键词 | PROKARYOTIC EXPRESSION ; GENE ; GCRV ; HOMEOSTASIS ; GENOTYPES ; ABUNDANT ; SPONGES ; PROTEIN ; PROFILE ; GENOME |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | MDPI AG |
WOS记录号 | WOS:000411963800156 |
资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; 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Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; 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Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of Institute of Hydrobiology, Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/32701] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Wang, Yaping |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | He, Libo,Zhang, Aidi,Xiong, Lv,et al. Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(9):16. |
APA | He, Libo.,Zhang, Aidi.,Xiong, Lv.,Li, Yongming.,Huang, Rong.,...&Wang, Yaping.(2017).Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(9),16. |
MLA | He, Libo,et al."Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.9(2017):16. |
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