Chemical fingerprint analysis of rhizomes of Gymnadenia conopsea by HPLC-DAD-MSn

	Chemical fingerprint analysis of rhizomes of Gymnadenia conopsea by HPLC-DAD-MSn
	Cai, M ; Zhou, Y ; Gesang, SL ; Bianba, CR ; Ding, LS
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2006
卷号	844 期号:2 页码:301_307
关键词	chemical fingerprint HPLC-DAD-MSn identification succinate derivative ester Gymnadenia conopsea
ISSN号	1570-0232
通讯作者	Ding, LS, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China.
产权排序	1
中文摘要	A high-performance liquid chromatography-diode array detection-tandem mass spectrometry (HPLC-DAD-MSn) method has been firstly developed for chemical fingerprint analysis of rhizomes of Gymnadenia conopsea R. Br. and rapid identification of major compounds in the fingerprints. Comparing the UV and MS spectra with those of reference compounds, seven main peaks in the fingerprints were identified as adenosine (1), 4-hydroxybenzyl alcohol (2), 4-hydroxybenzyl aldehyde (3), dactylorhin B (4), loroglossin (5), dactylorhin A (6) and militarine (7). Compounds 4-7 were succinate derivative esters and firstly discovered from this species. The Computer Aided Similarity Evaluation System (CASES) for chromatographic fingerprint of traditional Chinese medicine was employed to evaluate the similarities of 10 samples of the rhizomes of G. conopsea collected from Sichuan, Qinghai and Hebei provinces, Tibet autonomous region of China, and Nepal. These samples from different sources had similar chemical fingerprints. This method is specific and may serve for quality identification and comprehensive evaluation of this traditional Tibetan remedy. (c) 2006 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods; Chemistry, Analytical
收录类别	SCI
语种	英语
WOS记录号	WOS:000242843900014
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/17771]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Cai, M,Zhou, Y,Gesang, SL,et al. Chemical fingerprint analysis of rhizomes of Gymnadenia conopsea by HPLC-DAD-MSn[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,844(2):301_307.
APA	Cai, M,Zhou, Y,Gesang, SL,Bianba, CR,&Ding, LS.(2006).Chemical fingerprint analysis of rhizomes of Gymnadenia conopsea by HPLC-DAD-MSn.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,844(2),301_307.
MLA	Cai, M,et al."Chemical fingerprint analysis of rhizomes of Gymnadenia conopsea by HPLC-DAD-MSn".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 844.2(2006):301_307.