Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 广东省海洋药物重点实验室

	Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture
	Chen, F; sfchen@hkusua.hku.hk; Ip, PF
刊名	PROCESS BIOCHEMISTRY
	2005
卷号	40 期号:11 页码:3595-3599
关键词	Chlorella Zofingiensis Heterotrophic Culture Reactive Nitrogen Species Reactive Nitrogen Intermediates Astaxanthin
ISSN号	1359-5113
英文摘要	The fast growing unicellular green microalga Chlorella zofingiensis has attracted interest as a promising. organism for commercial production of the high-value carotenoid astaxanthin [Del Campo JA, Rodriguez H, Moreno J, Vargas MA, Rivas J, Guerrero MG. Accumulation of astaxanthin and lutein in Chlorella zofingiensis. Appl Microbiol Biotechnol 2004;64:848-54; Ip PR Wong KH, Chen F. Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture. Process Biochem 2004;39:1761-6; Ip PF, Chen, F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark. Process Biochem 2005;40:733-8.]. In this study, the reactive nitrogen species (RNS) peroxynitrite and the reactive nitrogen intermediate (RNI) nitryl chloride were applied to induce carotenogenesis leading to enhanced astaxanthin formation in C. zofingiensis in dark heterotrophic culture. Both the RNS and RNI when applied at an appropriate concentration stimulated astaxanthin biosynthesis in C. zofingiensis. Cultures treated with 1 mM peroxynitrite led to an enhanced yield of astaxanthin from 9.9 to 11.78 mg 1(-1). Nitryl chloride generated from the reaction mixture of 0.1 mM sodium nitrite with 0.5 mM sodium bypochlorite also increased the astaxanthin yield to 10.99 mg 1(-1). The present study is the first report demonstrating the possibility of using RNS and RNI for the enhancement of astaxanthin production in heterotrophic microalgal culture. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
资助项目	广东省海洋药物重点实验室
WOS记录号	WOS:000232393700029
公开日期	2011-06-29
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/2223]
专题	南海海洋研究所_广东省海洋药物重点实验室
通讯作者	sfchen@hkusua.hku.hk
推荐引用方式 GB/T 7714	Chen, F,sfchen@hkusua.hku.hk,Ip, PF. Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture[J]. PROCESS BIOCHEMISTRY,2005,40(11):3595-3599.
APA	Chen, F,sfchen@hkusua.hku.hk,&Ip, PF.(2005).Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture.PROCESS BIOCHEMISTRY,40(11),3595-3599.
MLA	Chen, F,et al."Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture".PROCESS BIOCHEMISTRY 40.11(2005):3595-3599.