Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)

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	Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)
	Hang, YH; Wei, JG; Guo, ML; Ji, HS; qinqw@scsio.ac.cn; Yan, Y; Ouyang, ZL; Huang, XH; Qin, QW
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012
卷号	33 期号:4 页码:916-925
关键词	Translationally Controlled Tumor Protein (Tctp) Epinephelus Coioides Sgiv Intracellular Localization Viral Replication
ISSN号	1050-4648
英文摘要	Translationally controlled tumor protein (TCTP) is an important molecule involved in multiple biological processes, such as cell growth, cell cycle progression, malignant transformation, and enhancement of the anti-apoptotic activity. In this study, the TCTP from orange-spotted grouper Epinephelus coioides (Ec-TCTP) was cloned and characterized. The full-length cDNA of Ec-TCTP was comprised of 1057 bp with a 510 bp open reading frame that encodes a putative protein of 170 amino acids. Recombinant Ec-TCTP (rEc-TCTP) was expressed in Escherichia BL21 (DE3) and purified for mouse anti-Ec-TCTP serum preparation. The rEc-TCTP fusion protein was demonstrated to possess antioxidant activity, which conferred resistance to H2O2 damage. Quantitative real-time PCR analysis revealed that Ec-TCTP mRNA is predominately expressed in the liver, and the expression was up-regulated in the liver of grouper after viral challenge with Singapore grouper iridovirus (SGIV). Intracellular localization revealed that Ec-TCTP expression was distributed predominantly in the cytoplasm. Although human TCTP has a role in apoptosis regulation, it is not known if grouper TCTP has any role in apoptosis regulation. Strikingly, grouper TCTP, when overexpressed in fathead minnow (FHM) cells, protected them from cell death induced by cycloheximide (CHX). In addition, overexpressed Ec-TCTP in grouper spleen (GS) cells inhibited the replication of SGIV. These results suggest that Ec-TCTP may play a critical role in their response to SGIV infection, through regulation of a cell death pathway that is common to fish and humans. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助项目	LMB
语种	英语
WOS记录号	WOS:000310185800028
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9784]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	qinqw@scsio.ac.cn
推荐引用方式 GB/T 7714	Hang, YH,Wei, JG,Guo, ML,et al. Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):916-925.
APA	Hang, YH.,Wei, JG.,Guo, ML.,Ji, HS.,qinqw@scsio.ac.cn.,...&Qin, QW.(2012).Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV).FISH & SHELLFISH IMMUNOLOGY,33(4),916-925.
MLA	Hang, YH,et al."Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):916-925.