Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement

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	Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement
	Zhang, Y; He, LS; Xu, Y; Matsumura, K; Zhang, G; Qi, SH; Qian, PY; boqianpy@ust.hk
刊名	PLOS ONE
	2012
卷号	7 期号:10 页码:e47195
ISSN号	1932-6203
英文摘要	The barnacle Balanus (= Amphibalanus) amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK) from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK) was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.
学科主题	Multidisciplinary Sciences
资助项目	LMB
语种	英语
WOS记录号	WOS:000310310200029
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9764]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	boqianpy@ust.hk
推荐引用方式 GB/T 7714	Zhang, Y,He, LS,Xu, Y,et al. Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement[J]. PLOS ONE,2012,7(10):e47195.
APA	Zhang, Y.,He, LS.,Xu, Y.,Matsumura, K.,Zhang, G.,...&boqianpy@ust.hk.(2012).Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement.PLOS ONE,7(10),e47195.
MLA	Zhang, Y,et al."Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement".PLOS ONE 7.10(2012):e47195.