An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua

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	An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua
	Zhang, S; wjli@ynu.edu.cn; Li, WJ; Li, J; Zhao, GZ; Varma, A; Qin, S; Xiong, Z; Huang, HY; Zhu, WY
刊名	PLOS ONE
	2012
卷号	7 期号:12 页码:-
ISSN号	1932-6203
英文摘要	Endophytic actinobacteria colonize internal tissues of their host plants and are considered as a rich and reliable source of diverse species and functional microorganisms. In this study, endophytic actinobacterial strain YIM 63111 was isolated from surface-sterilized tissue of the medicinal plant Artemisia annua. We identified strain YIM 63111 as a member of the genus Pseudonocardia. A. annua seedlings grown under both sterile and greenhouse conditions were inoculated with strain YIM 63111. The growth of A. annua seedlings was strongly reduced when YIM 63111 was inoculated at higher concentrations under sterile conditions. However, no growth inhibition was observed when A. annua was grown under greenhouse conditions. Using an enhanced green fluorescent protein (EGFP) expressing YIM 63111 strain, we also observed the endophytic colonization of A. annua seedling using confocal laser-scanning microscopy. The transcription levels of the key genes involved in artemisinin biosynthesis were investigated using real time RT-PCR, revealing that cytochrome P450 monooxygenase (CYP71AV1) and cytochrome P450 oxidoreductase (CPR) expression were up-regulated in A. annua upon inoculation with strain YIM 63111 under certain conditions. The up-regulation of these genes was associated with the increased accumulation of artemisinin. These results suggest that endophytic actinobacteria effectively stimulate certain plant defense responses. Our data also demonstrate the use of Pseudonocardia sp. strain YIM 63111 as a promising means to enhance artemisinin production in plants.
学科主题	Multidisciplinary Sciences
资助项目	LMB
语种	英语
WOS记录号	WOS:000313236200103
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9730]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	wjli@ynu.edu.cn
推荐引用方式 GB/T 7714	Zhang, S,wjli@ynu.edu.cn,Li, WJ,et al. An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua[J]. PLOS ONE,2012,7(12):-.
APA	Zhang, S.,wjli@ynu.edu.cn.,Li, WJ.,Li, J.,Zhao, GZ.,...&Xu, LH.(2012).An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua.PLOS ONE,7(12),-.
MLA	Zhang, S,et al."An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua".PLOS ONE 7.12(2012):-.