Identification and characterization of a native epitope common to norovirus strains GII/4, GII/7 and GII/8 | |
Qiu, HL; Li, X; Zhou, R; Wang, YS; Sheng, HY; Tian, XG; Li, HT; xiao_2005@hotmail.com; zhou3218@yahoo.com; yswang@scsio.ac.cn | |
刊名 | VIRUS RESEARCH |
2009 | |
卷号 | 140期号:40545页码:188-193 |
关键词 | Norovirus Capsid Protein Monoclonal Antibodies Epitope |
ISSN号 | 0168-1702 |
英文摘要 | Norovirus is an important cause of acute non-bacterial gastroenteritis in humans. The norovirus genus is comprised of at least five genogroups based on sequence differences. The norovirus genogroup II (GII/4) strain is recognized as the predominant genotype worldwide. We expressed a 60 kDa full-length recombinant capsid protein of norovirus GII/4 in Escherichia coli and generated three monoclonal antibodies (MAbs) against it. Western blotting indicated that all three MAbs had reactivity against the recombinant capsid protein and a 58 kDa native capsid protein of norovirus obtained from stool samples. MAb-capture ELISA showed that MAb detected segmental strains within GII antigens in clinical material. To identify the existent range of this epitope, epitope analyses were processed by expressing 12 amino acids of the GST-fusion peptides. The epitope analyses revealed that the MAb N2C3 recognized a continuous native epitope (WIRNNF60)-W-55 in the shell domain, which not only belongs to strain GII/4, but also to strains GII/7 and GII/8. This is a new native epitope to be reported for norovirus GII/4. (C) 2008 Elsevier B.V. All rights reserved. |
学科主题 | Virology |
URL标识 | 查看原文 |
资助项目 | LMB |
WOS记录号 | WOS:000264650600025 |
公开日期 | 2011-07-03 |
内容类型 | 期刊论文 |
源URL | [http://ir.scsio.ac.cn/handle/344004/5136] |
专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
通讯作者 | xiao_2005@hotmail.com; zhou3218@yahoo.com; yswang@scsio.ac.cn; shy-gir4020@163.com; tianxingui7902@yahoo.com.cn; lht05l02000@yahoo.com.cn; qtyunying@yahoo.com |
推荐引用方式 GB/T 7714 | Qiu, HL,Li, X,Zhou, R,et al. Identification and characterization of a native epitope common to norovirus strains GII/4, GII/7 and GII/8[J]. VIRUS RESEARCH,2009,140(40545):188-193. |
APA | Qiu, HL.,Li, X.,Zhou, R.,Wang, YS.,Sheng, HY.,...&qtyunying@yahoo.com.(2009).Identification and characterization of a native epitope common to norovirus strains GII/4, GII/7 and GII/8.VIRUS RESEARCH,140(40545),188-193. |
MLA | Qiu, HL,et al."Identification and characterization of a native epitope common to norovirus strains GII/4, GII/7 and GII/8".VIRUS RESEARCH 140.40545(2009):188-193. |
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