Separation of flavanone enantiomers and flavanone glucoside diastereomers from Balanophora involucrata Hook. f. by capillary electrophoresis and reversed-phase high-performance liquid chromatography on a C-18 column

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	Separation of flavanone enantiomers and flavanone glucoside diastereomers from Balanophora involucrata Hook. f. by capillary electrophoresis and reversed-phase high-performance liquid chromatography on a C-18 column
	Zhou, Y; Pan, JY; Zhang, S; Yan, LS; Tai, JD; Xiao, Q; Zou, K; Wu, J; zhsimd@scsio.ac.cn
刊名	JOURNAL OF CHROMATOGRAPHY A
	2008
卷号	1185 期号:1 页码:117-129
关键词	Balanophora Involucrata Flavanone Enantiomer Flavanone Glucoside Diastereomer Enantioseparation Diastereomeric Separation Capillary Electrophoresis High-performance Liquid Chromatography
ISSN号	0021-9673
英文摘要	A pair of flavanone glucoside diastereomers, (2R)- and (2S)-eriodictyol-5-O-beta-D-glucopyranoside (1a, 1b), was successfully separated by RP-C18 high-performance liquid chromatography from Balanophora involucrata Hook. f. Some other compounds, including a pair of flavanone enantiomers, (2R)- and (2S)-eriodictyol (2a, 2b), and a pair of flavanone glucoside diastereomers, (2R)- and (2S)-eriodictyol-7-O-beta-D-glucopyranoside(3a, 3b), were separated by capillary electrophoresis from the same plant. The absolute configurations at C-2 of la and 1b were determined based on their circular dichroism spectra. Enzymatic hydrolysis of 1a and 1b by beta-D-glucosidase afforded (2R)- and (2S)-eriodictyol, respectively, which were used as the authentic standards for co-elution to determine the migration order of the enantiomers, 2a and 2b. We also report the first example of identifying the migration order of 2a and 2b and resolving the separation of 3a and 3b by capillary electrophoresis. In addition, la was unambiguously characterized for the first time by NMR spectra. (C) 2008 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods ; Chemistry, Analytical
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000254322700015
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5004]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	zhsimd@scsio.ac.cn
推荐引用方式 GB/T 7714	Zhou, Y,Pan, JY,Zhang, S,et al. Separation of flavanone enantiomers and flavanone glucoside diastereomers from Balanophora involucrata Hook. f. by capillary electrophoresis and reversed-phase high-performance liquid chromatography on a C-18 column[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1185(1):117-129.
APA	Zhou, Y.,Pan, JY.,Zhang, S.,Yan, LS.,Tai, JD.,...&zhsimd@scsio.ac.cn.(2008).Separation of flavanone enantiomers and flavanone glucoside diastereomers from Balanophora involucrata Hook. f. by capillary electrophoresis and reversed-phase high-performance liquid chromatography on a C-18 column.JOURNAL OF CHROMATOGRAPHY A,1185(1),117-129.
MLA	Zhou, Y,et al."Separation of flavanone enantiomers and flavanone glucoside diastereomers from Balanophora involucrata Hook. f. by capillary electrophoresis and reversed-phase high-performance liquid chromatography on a C-18 column".JOURNAL OF CHROMATOGRAPHY A 1185.1(2008):117-129.