Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea

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	Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea
	Sun, W; Dai, SK; Jiang, SM; Wang, GH; Liu, GH; Wu, HB; Li, X; wuhoubo@scsio.ac.cn; lixiang@scsio.ac.cn
刊名	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
	2010
卷号	98 期号:1 页码:65-75
关键词	Actinobacteria Diversity Marine Sponge Salinispora
ISSN号	0003-6072
英文摘要	In this report, the diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve collected from a remote island of the South China Sea was investigated employing classical cultivation and characterization, 16S rDNA library construction, 16S rDNA-restriction fragment length polymorphism (rDNA-RFLP) and phylogenetic analysis. A total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phylogenetic analysis on the basis of their 16S rRNA gene sequences. Results showed that the 24 isolates were assigned to six genera including Salinispora, Gordonia, Mycobacterium, Nocardia, Rhodococcus and Streptomyces. This is the first report that Salinispora is present in a marine sponge from the South China Sea. Subsequently, 26 rDNA clones were selected from 191 clones in an Actinobacteria-specific 16S rDNA library of the H. perleve sample, using the RFLP technique for sequencing and phylogenetic analysis. In total, 26 phylotypes were clustered in eight known genera of Actinobacteria including Mycobacterium, Amycolatopsis, Arthrobacter, Brevibacterium, Microlunatus, Nocardioides, Pseudonocardia and Streptomyces. This study contributes to our understanding of actinobacterial diversity in the marine sponge H. perleve from the South China Sea.
学科主题	Microbiology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000276902800007
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3850]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	wuhoubo@scsio.ac.cn; lixiang@scsio.ac.cn
推荐引用方式 GB/T 7714	Sun, W,Dai, SK,Jiang, SM,et al. Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2010,98(1):65-75.
APA	Sun, W.,Dai, SK.,Jiang, SM.,Wang, GH.,Liu, GH.,...&lixiang@scsio.ac.cn.(2010).Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,98(1),65-75.
MLA	Sun, W,et al."Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 98.1(2010):65-75.