The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells

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	The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells
	Luo, P; Zhao, Z; Chen, C; Hu, CQ; Ren, CH; Zhao, JJ; Zhang, LP; Jiang, XA; cqhu@scsio.ac.cn; Wang, QB
刊名	MICROBIOLOGY-SGM
	2010
卷号	156 页码:2864-2872
ISSN号	1350-0872
英文摘要	Vibrio alginolyticus is a Gram-negative bacterium and has been recognized as an opportunistic pathogen in humans as well as marine animals. However, the virulence mechanisms for this species of Vibrio have not been elucidated. This study characterized multiple mechanisms that induce cell death in fish cells upon infection with a V. alginolyticus strain, ZJO. The bacterium required its type III secretion system (T3SS) to cause rapid death of infected fish cells. Dying cells exhibited some features of apoptotic cells, such as membrane blebbing, nuclear condensation and DNA fragmentation. Further studies showed that caspase-3 was activated by the T3SS of the ZJO strain, confirming that infection with V. alginolyticus rapidly induces T3SS-dependent apoptosis in fish cells. Infection with the ZJO strain also led to membrane pore formation and release of cellular contents from infected fish cells, as evidenced by lactate dehydrogenase release and the uptake of a membrane-impermeable dye. Importantly, inhibition of apoptosis did not prevent ZJO-infected cells from releasing cellular contents and did not block cell rounding. Taken together, these data demonstrate that infection with V. alginolyticus may promote at least three different T3SS-dependent events, which lead to the death of fish cells. This study provides an important insight into the mechanism used by Vibrio species to cause host-cell death.
学科主题	Microbiology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000282565700026
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3754]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	cqhu@scsio.ac.cn
推荐引用方式 GB/T 7714	Luo, P,Zhao, Z,Chen, C,et al. The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells[J]. MICROBIOLOGY-SGM,2010,156:2864-2872.
APA	Luo, P.,Zhao, Z.,Chen, C.,Hu, CQ.,Ren, CH.,...&Wang, QB.(2010).The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells.MICROBIOLOGY-SGM,156,2864-2872.
MLA	Luo, P,et al."The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells".MICROBIOLOGY-SGM 156(2010):2864-2872.