Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets

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	Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets
	Hu, CQ; cqhu@scsio.ac.cn; Chen, C; Zhang, LP; Luo, P; Feng, JB
刊名	AQUACULTURE RESEARCH
	2010
卷号	41 期号:12 页码:1778-1790
关键词	Yellow Grouper Microbiota Dgge Skin Gill Intestinal-non-adherent Bacteria Intestinal-adherent Bacteria
ISSN号	1355-557X
英文摘要	The rapidly growing yellow grouper industry has experienced relatively severe bacterial disease problems in China. The proliferation of pathogens in fish can be suppressed by commensal microbiota. In this background, we used nested polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis to investigate microbiota in the skin, gills and intestines, including adherent bacteria and non-adherent bacteria in yellow grouper fed with natural diet and complete feed. A total of 21 bacterial species were identified using phylogenetic analysis. The gamma-Proteobacteria group (81.0%, 17 species) dominated the bacterial communities in yellow grouper completely. Others belonged to Firmicutes (9.5%, two species), Actinobacteria (4.75%, one species) and Verrucomicrobia (4.75%, one species). The higher similarities (above 91%) of the DGGE band patterns in skin, gill and intestinal-non-adherent bacteria between two groups of fish indicated that existed more stable microbial communities existed in these specifically ecological niches in yellow grouper. However, considerable differences existed between two intestinal-adherent bacteria (IAB) samples; that is, compared with natural diet fed yellow grouper, higher bacterial apparent species richness and possibly less abundance existed in IAB in fish fed with complete diets, probably indicating that the community structures in IAB were affected easily and significantly by diet.
学科主题	Fisheries
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000283946200006
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3732]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	cqhu@scsio.ac.cn
推荐引用方式 GB/T 7714	Hu, CQ,cqhu@scsio.ac.cn,Chen, C,et al. Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets[J]. AQUACULTURE RESEARCH,2010,41(12):1778-1790.
APA	Hu, CQ,cqhu@scsio.ac.cn,Chen, C,Zhang, LP,Luo, P,&Feng, JB.(2010).Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets.AQUACULTURE RESEARCH,41(12),1778-1790.
MLA	Hu, CQ,et al."Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets".AQUACULTURE RESEARCH 41.12(2010):1778-1790.