Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host

doi:10.3390/md17020127

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host
	Ju, Jianhua 2; Shao, Mingwei 2; Ma, Juying 2; Li, Qinglian 2
刊名	MARINE DRUGS
	2019
卷号	17 期号:2 页码:127
关键词	Streptomyces sp SCSIO ZS0098 aborycin heterogenous expression antibacterial activity
ISSN号	1660-3397
DOI	10.3390/md17020127
英文摘要	Aborycin is a ribosomally synthesized member of the type I lasso peptide natural products. In the present study, aborycin was isolated and identified from the deep-sea-derived microbe Streptomyces sp. SCSIO ZS0098. The aborycin biosynthetic gene cluster (abo) was identified on the basis of genome sequence analyses and then heterologously expressed in Streptomyces coelicolor M1152 to effectively produce aborycin. Aborycin generated in this fashion exhibited moderate antibacterial activity against 13 Staphylococcus aureus strains from various sources with minimum inhibitory concentrations MICs = 8.0 128 mu g/mL, against Enterococcus faecalis ATCC 29212 with an MIC = 8.0 mu g/mL, and against Bacillus thuringiensis with MIC = 2.0 mu g/mL. Additionally, aborycin displayed potent antibacterial activity (MIC = 0.5 mu g/mL) against the poultry pathogen Enterococcus gallinarum 5F52C. The reported abo cluster clearly has the potential to provide a means of expanding the repertoire of anti-infective type I lasso peptides.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17905]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 110039, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol,RNAM Ctr Marine Micr, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Ju, Jianhua,Shao, Mingwei,Ma, Juying,et al. Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host[J]. MARINE DRUGS,2019,17(2):127.
APA	Ju, Jianhua,Shao, Mingwei,Ma, Juying,&Li, Qinglian.(2019).Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host.MARINE DRUGS,17(2),127.
MLA	Ju, Jianhua,et al."Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host".MARINE DRUGS 17.2(2019):127.