Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy | |
Min, Junwei1,2; Yao, Baoli2; Trendafilova, Veselina1; Ketelhut, Steffi1; Kastl, Lena1; Greve, Burkhard3; Kemper, Bjoern1 | |
刊名 | JOURNAL OF BIOPHOTONICS |
2019-09 | |
卷号 | 12期号:9 |
关键词 | autofocusing digital holographic microscopy flow cytometry image flow cytometry label-free cytometry quantitative microscopy quantitative phase imaging |
ISSN号 | 1864-063X;1864-0648 |
DOI | 10.1002/jbio.201900085 |
产权排序 | 1 |
英文摘要 | We combined Michelson-interferometer-based off-axis digital holographic microscopy (DHM) with a common flow cytometry (FCM) arrangement. Utilizing object recognition procedures and holographic autofocusing during the numerical reconstruction of the acquired off-axis holograms, sharply focused quantitative phase images of suspended cells in flow were retrieved without labeling, from which biophysical cellular features of distinct cells, such as cell radius, refractive index and dry mass, can be subsequently retrieved in an automated manner. The performance of the proposed concept was first characterized by investigations on microspheres that were utilized as test standards. Then, we analyzed two types of pancreatic tumor cells with different morphology to further verify the applicability of the proposed method for quantitative live cell imaging. The retrieved biophysical datasets from cells in flow are found in good agreement with results from comparative investigations with previously developed DHM methods under static conditions, which demonstrates the effectiveness and reliability of our approach. Our results contribute to the establishment of DHM in imaging FCM and prospect to broaden the application spectrum of FCM by providing complementary quantitative imaging as well as additional biophysical cell parameters which are not accessible in current high-throughput FCM measurements. |
语种 | 英语 |
出版者 | WILEY-V C H VERLAG GMBH |
WOS记录号 | WOS:000486778200017 |
内容类型 | 期刊论文 |
源URL | [http://ir.opt.ac.cn/handle/181661/31882] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Kemper, Bjoern |
作者单位 | 1.Univ Munster, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany 2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Shaanxi, Peoples R China 3.Univ Hosp Muenster, Dept Radiotherapy Radiooncol, Munster, Germany |
推荐引用方式 GB/T 7714 | Min, Junwei,Yao, Baoli,Trendafilova, Veselina,et al. Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy[J]. JOURNAL OF BIOPHOTONICS,2019,12(9). |
APA | Min, Junwei.,Yao, Baoli.,Trendafilova, Veselina.,Ketelhut, Steffi.,Kastl, Lena.,...&Kemper, Bjoern.(2019).Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy.JOURNAL OF BIOPHOTONICS,12(9). |
MLA | Min, Junwei,et al."Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy".JOURNAL OF BIOPHOTONICS 12.9(2019). |
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