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	Long-Term Field Fertilization Significantly Alters Community Structure of Ammonia-Oxidizing Bacteria rather than Archaea in a Paddy Soil
	Wu, Yucheng; Lu, Lu; Wang, Baozhan; Lin, Xiangui; Zhu, Jianguo; Cai, Zucong; Yan, Xiaoyuan; Jia, Zhongjun
刊名	SOIL SCIENCE SOCIETY OF AMERICA JOURNAL
	2011-07-01
卷号	75期号:4页码:1431-1439
ISSN号	0361-5995
DOI	10.2136/sssaj2010.0434
通讯作者	Jia, Zhongjun(jia@issas.ac.cn)
英文摘要	Long-term field fertilization experiments may provide useful insight into the relative contributions of NH(3)-oxidizing bacteria (AOB) and NH(3)-oxidizing archaea (AOA) to soil nitrification. In this study, the abundance and composition of AOB and AOA were investigated in bulk soil from paddy field plots (4 by 5 m) that received no fertilization (CK), 180 kg urea N ha(-1) yr(-1) (NPK), or 180 kg urea N plus 4500 kg rice (Oryza sativa L.) straw ha(-1) yr(-1) (NPK./OM) since 1988 (each with three replicated plots). Our results clearly indicate that long-term fertilization (22 yr) significantly altered the community structure of AOB rather than AOA in the soil within fertilized plots compared with the CK plot. Quantitative polymerase chain reaction analysis of the amoA genes that encode the ammonia monooxygenase subunit A revealed that the AOB in the fertilized plots were 50 times more abundant than in the CK plot. Denaturing gradient gel electrophoresis fingerprinting of the amoA genes demonstrated a drastic shift in the AOB community structure, resulting in highly enriched AOB similar to Nitrosospira cluster 3 in the fertilized plots. In contrast, the population size and composition of the AOA community remained largely unchanged in all plots. Furthermore, a positive correlation was observed between the bacterial rather than archaeal amoA gene copy numbers and potential nitrification activity among the soils. The distinct responses of AOA and AOB to long-term field fertilization were probably related to the availability of NH(3). Our results suggest that AOB might play major roles in the NH(3) oxidation that occurs in bulk soil under the in situ fertilized paddy field conditions tested in this study.
收录类别	SCI
WOS关键词	MICROCOSM EXPERIMENTS ; SANDY LOAM ; RICE FIELD ; NITRIFICATION ; OXIDATION ; CRENARCHAEOTA ; RHIZOSPHERE ; CULTIVATION ; DIVERSITY ; RELEVANCE
WOS研究方向	Agriculture
WOS类目	Soil Science
语种	英语
出版者	SOIL SCI SOC AMER
WOS记录号	WOS:000293076400024
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2559004
专题	南京土壤研究所
通讯作者	Jia, Zhongjun
作者单位	Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China
推荐引用方式 GB/T 7714	Wu, Yucheng,Lu, Lu,Wang, Baozhan,et al. Long-Term Field Fertilization Significantly Alters Community Structure of Ammonia-Oxidizing Bacteria rather than Archaea in a Paddy Soil[J]. SOIL SCIENCE SOCIETY OF AMERICA JOURNAL,2011,75(4):1431-1439.
APA	Wu, Yucheng.,Lu, Lu.,Wang, Baozhan.,Lin, Xiangui.,Zhu, Jianguo.,...&Jia, Zhongjun.(2011).Long-Term Field Fertilization Significantly Alters Community Structure of Ammonia-Oxidizing Bacteria rather than Archaea in a Paddy Soil.SOIL SCIENCE SOCIETY OF AMERICA JOURNAL,75(4),1431-1439.
MLA	Wu, Yucheng,et al."Long-Term Field Fertilization Significantly Alters Community Structure of Ammonia-Oxidizing Bacteria rather than Archaea in a Paddy Soil".SOIL SCIENCE SOCIETY OF AMERICA JOURNAL 75.4(2011):1431-1439.

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