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	A gyrB-targeted PCR for Rapid Identification of Salmonella
	Ye, Xuhong2,3,4; Wang, Yiming2,3; Lin, Xiangui1,2,3
刊名	CURRENT MICROBIOLOGY
	2011-11-01
卷号	63期号:5页码:477-483
ISSN号	0343-8651
DOI	10.1007/s00284-011-0007-1
通讯作者	Lin, Xiangui(xglin@issas.ac.cn)
英文摘要	Salmonella causes the majority of infections in humans and homeothermic animals. This article describes a specific polymerase chain reaction (PCR) method developed for a rapid identification of Salmonella. A gyrB-targeted species-specific primer pair, S-P-for (5'-GGT GGT TTC CGT AAA AGT A-3') and S-P-rev (5'-GAA TCG CCT GGT TCT TGC-3'), was successfully designed. PCR with all the Salmonella strains produced a 366- bp DNA fragment that was absent from all the non-Salmonella strains tested. The detection limit of the PCR was 0.01 ng with genomic DNA or 3.2 cells per assay. Good specificity was also demonstrated by fecal samples, from which only the gyrB gene of Salmonella was amplified. Using the culture-PCR method, 27 isolates on Salmonella-Shigella (SS) medium were rapidly identified as Salmonella, which was confirmed by the sequencing of the gyrB gene.
收录类别	SCI
WOS关键词	POLYMERASE-CHAIN-REACTION ; ENTERITIDIS ; SEQUENCES ; GENE ; FOOD ; MICROORGANISMS ; HYBRIDIZATION ; PATHOGENS ; PROBE
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000295164200012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2558890
专题	南京土壤研究所
通讯作者	Lin, Xiangui
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Dept Biol & Biochem, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 3.Hongkong Baptist Univ, Joint Open Lab Soil & Environm, Nanjing 210008, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ye, Xuhong,Wang, Yiming,Lin, Xiangui. A gyrB-targeted PCR for Rapid Identification of Salmonella[J]. CURRENT MICROBIOLOGY,2011,63(5):477-483.
APA	Ye, Xuhong,Wang, Yiming,&Lin, Xiangui.(2011).A gyrB-targeted PCR for Rapid Identification of Salmonella.CURRENT MICROBIOLOGY,63(5),477-483.
MLA	Ye, Xuhong,et al."A gyrB-targeted PCR for Rapid Identification of Salmonella".CURRENT MICROBIOLOGY 63.5(2011):477-483.

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