| High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin | |
| Chen, Ximing1,2; Li, Juan1,2; Sun, Haili3; Li, Shiweng4; Chen, Tuo1,2; Liu, Guangxiu1,2; Dyson, Paul5 | |
| 刊名 | SCIENTIFIC REPORTS
 |
| 2017-11-06 | |
| 卷号 | 7页码:9 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-15149-3 |
| 通讯作者 | Liu, Guangxiu(liugx@lzb.ac.cn) |
| 英文摘要 | Drug resistance is a major problem in antibacterial chemotherapy. Apidaecins, which refer to a series of small, proline-rich antimicrobial peptides, are predominantly active against many drug-resistant bacteria. The apidaecins have special antibacterial mechanisms, and are non-toxic for human cells, a prerequisite for using them as novel antibiotic drugs. However, no efficient non-tagged apidaecin expression system has been reported, which is the limiting factor for their application. Here we successfully generated a Pichia pastoris transformant expressing and secreting apidaecin. However, expression was unstable and poor. Analysis of this revealed that the integration plasmid was frequently lost and that apidaecin expression resulted in cell death. Using N-methyl-N-nitro-N-nitroso-guanidine mutagenesis and selection, a mutant strain Apmu4 was derived, in which the rate of loss of the integration plasmid was much lower after induction, and which produced improved titres of apidaecin. Additionally, we discovered that using glucose as the sole carbon source to pre-culture the strain before induction could greatly enhance apidaecin production. A pilot-scale 10 L fermentation yielded 418 mg/L of recombinant apidaecin, which represents the highest reported yield of apidaecin. Consequently, this study reports the first super heterologous expression and secretion of apidaecin in yeast. |
| 收录类别 | SCI |
| WOS关键词 | ANTIMICROBIAL PEPTIDES ; PROTEIN-PRODUCTION ; KLUYVEROMYCES-LACTIS ; EXPRESSION ; ANTIBIOTICS ; SYSTEM ; ENTEROCIN ; BIODIVERSITY ; BACTERIOCIN ; SPECTRUM |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000414415000043 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2557645 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Liu, Guangxiu |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Desert & Desertificat, Lanzhou, Gansu, Peoples R China 2.Key Lab Extreme Environm Microbial Resources & En, Jiayuguan, Peoples R China 3.Lanzhou City Univ, Sch Chem & Environm Sci, Lanzhou, Gansu, Peoples R China 4.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Gansu, Peoples R China 5.Swansea Univ, Inst Life Sci, Med Sch, Singleton Pk, Swansea SA2 8PP, W Glam, Wales |
| 推荐引用方式 GB/T 7714 | Chen, Ximing,Li, Juan,Sun, Haili,et al. High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin[J]. SCIENTIFIC REPORTS,2017,7:9. |
| APA | Chen, Ximing.,Li, Juan.,Sun, Haili.,Li, Shiweng.,Chen, Tuo.,...&Dyson, Paul.(2017).High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin.SCIENTIFIC REPORTS,7,9. |
| MLA | Chen, Ximing,et al."High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin".SCIENTIFIC REPORTS 7(2017):9. |
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