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	MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells
	Wang, Jin; Li, Xinrong; Liu, Yubing; Zhao, Xin; Chen, Cuiyun; Tian, Fang
刊名	PLANT GROWTH REGULATION
	2011-04-01
卷号	63期号:3页码:207-216
关键词	Apoptosis Cytochrome c Hydrogen peroxide Mitogen-activated protein kinase Salt stress
ISSN号	0167-6903
DOI	10.1007/s10725-010-9517-2
通讯作者	Wang, Jin(wangjinlzb@hotmail.com)
英文摘要	Programmed cell death (PCD) is an active cellular suicide that occurs both in animals and plants throughout development and in response to abiotic or biotic stress. In contrast to plant hypersensitive response-like cell death, little is known about the molecular machinery that regulates the halophyte plant PCD under high salinity stress. Since mitogen-activated protein kinases (MAPKs) are involved in plant response/tolerance to salt stress, and plant MAPK genes belong to the extracellular signal-regulated kinase (ERK) subfamily, we have investigated the role of ERK-like enzymes in high salinity stress-induced cell death in Thellungiella halophila. The data showed that ERK-like enzymes were early (10 min) and transiently activated under 300 mM NaCl stress. Pretreatment with 10 mu M U0126, a special MEK/ERK inhibitor, resulted in a small but statistically significant increase of the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL)-positive nuclei in contrast to salt alone. The effects of U0126 on H(2)O(2) production and cytochrome c (cyt c) release were also investigated. We found that the pretreatment with U0126 accelerated H(2)O(2) production as well as cyt c release, and eventually enhanced cell death. The results suggest that ERK-like enzymes in Thellungiella halophila may act as a positive regulator of salt tolerance, as illustrated by pretreatment with U0126 which enhanced cell death under high salinity stress.
收录类别	SCI
WOS关键词	ACTIVATED PROTEIN-KINASE ; CYTOSOLIC ASCORBATE PEROXIDASE ; CASPASE-LIKE PROTEASES ; TOBACCO BY-2 CELLS ; ARABIDOPSIS-THALIANA ; OXIDATIVE STRESS ; POTASSIUM-TRANSPORT ; DISEASE RESISTANCE ; SIGNALING CASCADES ; MULTIPLE LEVELS
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000288562100001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2556468
专题	寒区旱区环境与工程研究所
通讯作者	Wang, Jin
作者单位	Chinese Acad Sci, Shapotou Desert Res & Expt Stn, Extreme Stress Resistance & Biotechnol Lab, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jin,Li, Xinrong,Liu, Yubing,et al. MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells[J]. PLANT GROWTH REGULATION,2011,63(3):207-216.
APA	Wang, Jin,Li, Xinrong,Liu, Yubing,Zhao, Xin,Chen, Cuiyun,&Tian, Fang.(2011).MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells.PLANT GROWTH REGULATION,63(3),207-216.
MLA	Wang, Jin,et al."MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells".PLANT GROWTH REGULATION 63.3(2011):207-216.

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