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	Isolation and expression analysis of two cold-inducible genes encoding putative CBF transcription factors from Chinese cabbage (Brassica pekinensis Rupr.)
	Zhang, Yong; Yang, Tong-Wen; Zhang, Li-Jing; Zhang, Teng-Guo; Di, Cui-Xia; Xu, Shi-Jian; An, Li-Zhe
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2006-07-01
卷号	48期号:7页码:848-856
关键词	Brassica pekinensis cold acclimation cold induced gene C-repeat/dehydration-responsive element binding factors (CBF/DREB1) expression pattern
ISSN号	1672-9072
通讯作者	An, Li-Zhe(lizhean@lzu.edu.cn)
英文摘要	Two homologous genes of the Arabidopsis C-repeat/dehydration-responsive element binding factors (CBF/DREB1) transcriptional activator were isolated by RT-PCR from Chinese cabbage (Brassica pekinensis Rupr. cv. inbai 5) and were designated as BcCBF1 and BcCBF2. Each encodes a putative CBF/DREB1 protein with an AP2 (Apetal2) DNA-binding domain, a putative nuclear localization signal, and a possible acidic activation domain. Deduced amino acid sequences show that BcCBF1 is very similar to the Arabidopsis CBF1, whereas BcCBF2 is different in that it contains two extra regions of 24 and 20 amino acids in the acidic domain. The mRNA accumulation profiles indicated that the expression of BcCBF1 and BcCBF2 is strongly induced by cold treatment, but does not respond similarly to dehydration or abscisic acid (ABA) treatment. However, the cold-induced accumulation of BcCBF2 mRNA was rapid but short-lived compared with that of BcCBF1. The mRNA levels of both BcCBF1 and BcCBF2 were higher in leaves than in roots when plants were exposed to cold, whereas, salt stress caused higher accumulation of BcCBF2 mRNA in roots than in leaves, suggesting that the organ specificity of the gene expression of the BcCBFs is probably stress dependent. In addition, the accumulation of BcCBF1 and BcCBF2 mRNAs was greatly enhanced by light compared with darkness when seedlings were exposed to cold. It is concluded that the two BcCBF proteins may be involved in the process of plant response to cold stress through an ABA-independent pathway and that there is also a cross-talk between the light signaling conduction pathway and the cold response pathway in B. pekinensis as in Arabidopsis.
收录类别	SCI
WOS关键词	FREEZING TOLERANCE ; LOW-TEMPERATURE ; SIGNAL-TRANSDUCTION ; ARABIDOPSIS-THALIANA ; CIRCADIAN CLOCK ; ABSCISIC-ACID ; DROUGHT ; ACCLIMATION ; STRESS ; ELEMENT
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000238913900012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2555815
专题	寒区旱区环境与工程研究所
通讯作者	An, Li-Zhe
作者单位	1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Arid & Grassland Agroecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yong,Yang, Tong-Wen,Zhang, Li-Jing,et al. Isolation and expression analysis of two cold-inducible genes encoding putative CBF transcription factors from Chinese cabbage (Brassica pekinensis Rupr.)[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2006,48(7):848-856.
APA	Zhang, Yong.,Yang, Tong-Wen.,Zhang, Li-Jing.,Zhang, Teng-Guo.,Di, Cui-Xia.,...&An, Li-Zhe.(2006).Isolation and expression analysis of two cold-inducible genes encoding putative CBF transcription factors from Chinese cabbage (Brassica pekinensis Rupr.).JOURNAL OF INTEGRATIVE PLANT BIOLOGY,48(7),848-856.
MLA	Zhang, Yong,et al."Isolation and expression analysis of two cold-inducible genes encoding putative CBF transcription factors from Chinese cabbage (Brassica pekinensis Rupr.)".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 48.7(2006):848-856.

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