OIL AND FAT HYDROLYSIS WITH LIPASE FROM ASPERGILLUS SP

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	OIL AND FAT HYDROLYSIS WITH LIPASE FROM ASPERGILLUS SP
	FU, X ; ZHU, X ; GAO, K ; DUAN, J
刊名	JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
	1995-05-01
卷号	72 期号:5 页码:527-531
关键词	Enzyme Concentration Enzyme Recovery Fatty Acids Lipase Lipase Activity Lipolysis Oil And Fat Percent Hydrolysis Reaction Time Specificity
ISSN号	0003-021X
英文摘要	Hydrolysis of olive oil, soybean oil, mink fat, lard, palm oil, coconut oil, and a hydrogenated, hardened oil with lipase from an Aspergillus sp. has been studied. The lipase had high specific activity (60,000 U/g) and did not show any positional specificity. The lipase proved to be a more effective catalyst than Lipolase from A. oryzae, with an optimal activity at 37 degrees C and pH 6.5-7.0. It was activated by Ca2+ but inactivated by organic solvents such as isopropanol and propanone. All substrates examined could be hydrolyzed to corresponding fatty acids with this enzyme at concentrations of 5-30 U/meq with yields of 90-99% in 2-24 h. The degree of hydrolysis was almost logarithmically linear with reaction time and occurred in two stages. The lipase was stable and could be repeatedly recycled for hydrolysis.
WOS研究方向	Chemistry ; Food Science & Technology
语种	英语
出版者	AMER OIL CHEMISTS SOC
WOS记录号	WOS:A1995QX92100005
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55721]
专题	沈阳应用生态研究所_沈阳应用生态研究所
作者单位	1.CHINESE ACAD SCI,SHENYANG INST APPL ECOL,SHENYANG 110015,PEOPLES R CHINA 2.DALIAN UNIV TECHNOL,DEPT FINE CHEM TECHNOL,DALIAN 116012,PEOPLES R CHINA
推荐引用方式 GB/T 7714	FU, X,ZHU, X,GAO, K,et al. OIL AND FAT HYDROLYSIS WITH LIPASE FROM ASPERGILLUS SP[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,1995,72(5):527-531.
APA	FU, X,ZHU, X,GAO, K,&DUAN, J.(1995).OIL AND FAT HYDROLYSIS WITH LIPASE FROM ASPERGILLUS SP.JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,72(5),527-531.
MLA	FU, X,et al."OIL AND FAT HYDROLYSIS WITH LIPASE FROM ASPERGILLUS SP".JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 72.5(1995):527-531.