Optimization of solid fermentation of cellulase from Trichoderma koningii

CORC > 沈阳应用生态研究所 > 中国科学院沈阳应用生态研究所 > 沈阳应用生态研究所

	Optimization of solid fermentation of cellulase from Trichoderma koningii
	Li, PJ; Jing, DB; Zhou, QX; Zhang, CG; Li, PJ, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2004
卷号	16 期号:5 页码:816-820
关键词	Trichoderma Koningii Substrate Prescription Fpa Cmcase Cotton Lyase Beta-glucosidase
ISSN号	1001-0742
英文摘要	To exploit peashrub resources in Ordos as fodders, it is very crucial to realize industrial production of cheap cellulase of high activity by optimizing culture technology, especially culture substrate. In this study, a new prescription experiment based on uniform design ideal was invented and successfully applied in the solid fermentation of Trichoderma koningii F244, which was performed with two different temperature degrees. The activities of FPA, cotton lyase, CMCase and P-glucosidase were assayed and then mathematical models of enzymatic activities, which were figured out by Unconstraint Mathematical Programming, were developed by Multivariate Regression Program of SPSS10.0. Enzymatic activities of optimized substrate prescriptions corresponding to mathematical models were forecasted to determine an ideal substrate prescription. It is revealed that in solid fermentation, Tween80 has negative effect on cellulase production. Furthermore, the ideal prescription for cellulase complex production by Trichoderma koningii F244 was straw powder 16.9%, wheat bran 26.5%, (NH4)(2)SO4 9.5% and water 47.1%, whose corresponding cellulase activity was expected to be at the same high level with that of Trichoderma reesei Q9414 on its own recommended substrate. Especially, goats mainly fed on peashrub tissues mixed with cellulase complex of this prescription and culture technology, got an incremental ratio of 0.3 kg/d, which brought a very promising feeding prospect for local peashrub resource. By populization of this cellulase complex, it can integrate living standard, economic construction of local residents into vegetational restoration tightly and thus this paper will be very meaningful to be use for reference for western China like Ordos to realize its sustainable development of economy, society and environment.
学科主题	Environmental Sciences
语种	英语
WOS记录号	WOS:000223687800024
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55257]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Li, PJ, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Li, PJ,Jing, DB,Zhou, QX,et al. Optimization of solid fermentation of cellulase from Trichoderma koningii[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2004,16(5):816-820.
APA	Li, PJ,Jing, DB,Zhou, QX,Zhang, CG,&Li, PJ, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2004).Optimization of solid fermentation of cellulase from Trichoderma koningii.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,16(5),816-820.
MLA	Li, PJ,et al."Optimization of solid fermentation of cellulase from Trichoderma koningii".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 16.5(2004):816-820.