A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)

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	A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)
	Lu, AG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China; Bai, XY; Ni, JF; Lu, AG; Wu, WF; Niu, RF
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2005
卷号	32 期号:4 页码:365-370
关键词	Diphtheria Toxin Vascular Endothelial Growth Factor (Vegf) Vascular Endothelial Growth Factor Receptor-1 Fusion Protein
ISSN号	1000-3282
英文摘要	Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae that has been lysogenized by P bacteriophage that carries the DT gene. It blocks protein synthesis and kills the target eukaryotic cell. The R82A, K84A, H86A mutant of vascular endothelial growth factor (VEGF) specially binds its receptor 1 (VEGFR-1) that is expressed highly on surface of tumor blood vessel. DT genomic DNA was extracted first and then the gene that coding the T domain and C domain of DT (DT391) were amplified. The R82A, K84A, H86A mutant were introduced to VEGF by site-directed mutagenesis. Then a VEGFR-I targeting fusion protein, DT391-mVEGF, was constructed by substituting the receptor binding domain of DT with the VEGF mutant which shows high affinity to a receptor expressed on tumor vascular. With DT391, a protein without the mVEGF domain of DT391-mVEGF, as a negative control in cytotoxicity assay, the hybrid protein DT391-mVEGF showed an inhibition to the growth of VEGFR-1 positive tumor cell.
学科主题	Biochemistry & Molecular Biology ; Biophysics
语种	中文
WOS记录号	WOS:000228316600013
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55161]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Lu, AG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Lu, AG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China,Bai, XY,Ni, JF,et al. A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(4):365-370.
APA	Lu, AG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China,Bai, XY,Ni, JF,Lu, AG,Wu, WF,&Niu, RF.(2005).A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1).PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(4),365-370.
MLA	Lu, AG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China,et al."A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.4(2005):365-370.