Optimization of cellulase complex formulation for peashrub biomass hydrolysis

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	Optimization of cellulase complex formulation for peashrub biomass hydrolysis
	Jing, DB; Li, PJ; Xiong, XZ; Wang, LH; Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2007
卷号	75 期号:4 页码:793-800
关键词	Simultaneous Saccharification And Fermentation Cellulase-inoculant Mixture Peashrub Woody Biomass Crude Protein Crude Fiber Lactobacillus Spp
ISSN号	0175-7598
英文摘要	To improve efficiency and reduce cost, solid state simultaneous saccharification and fermentation of peashrub woody biomass was investigated under anaerobic conditions at 50 degrees C, with a cellulase-inoculant mixture consisting of Trichoderma koningii cellulase, Aspergillus niger cellulase, and Lactobacillus. Experimental formulations were prepared according to uniform prescription design principles. By crude protein, crude fiber models constructed using multivariate regression in SPSS and solutions analysis through unconstrained mathematical optimization in Microsoft Excel, it was clearly revealed that low pH value (3.8) from lactic acid accumulation produced by Lactobacillus would ultimately limit enzymatic hydrolysis during long-term fermentation (30 days). It was shown that a cellulase complex with filter paper cellulase/carboxymethyl cellulase/cotton lyase/beta-glucosidase/pectinase of activity ratios of 0.6:1:0.3:1:2.6 could effectively break peashrub cell wall structure by biodegradation of easily digested components and, then, release cellular contents to improve crude protein content. Thus, the enzymatic hydrolysis of peashrub biomass by the optimized cellulase complex could improve crude protein content by 45.3% (from 8.45 to 12.28%), although it only biodegraded about 10.90% of the crude fiber (from 44.45 to 40.08%).
学科主题	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000248049100009
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54643]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China
推荐引用方式 GB/T 7714	Jing, DB,Li, PJ,Xiong, XZ,et al. Optimization of cellulase complex formulation for peashrub biomass hydrolysis[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(4):793-800.
APA	Jing, DB,Li, PJ,Xiong, XZ,Wang, LH,&Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China.(2007).Optimization of cellulase complex formulation for peashrub biomass hydrolysis.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,75(4),793-800.
MLA	Jing, DB,et al."Optimization of cellulase complex formulation for peashrub biomass hydrolysis".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 75.4(2007):793-800.