Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination

doi:10.1021/acs.est.8b05871

	Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination
	Yin, Yongchao 1,2,3; Yan, Jun 2,6; Chen, Gao 3,4; Murdoch, Fadime Kara 1,2,3; Pfisterer, Nina 2,3,7; Loffler, Frank E.1,2,3,4,5
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2019-01-15
卷号	53 期号:2 页码:692-701
ISSN号	0013-936X
DOI	10.1021/acs.est.8b05871
英文摘要	Organohalide-respiring bacteria are key players for the turnover of organohalogens. At sites impacted with chlorinated ethenes, bioremediation promotes reductive dechlorination; however, stoichiometric conversion to environmentally benign ethene is not always achieved. We demonstrate that nitrous oxide (N2O), a compound commonly present in groundwater, inhibits organohalide respiration. N2O concentrations in the low micromolar range decreased dechlorination rates and resulted in incomplete dechlorination of tetrachloroethene (PCE) in Geobacter lovleyi strain SZ and of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC) in Dehalococcoides mccartyi strain BAV1 axenic cultures. Presumably, N2O interferes with reductive dechlorination by reacting with super-reduced Co(I)-corrinoids of reductive dehalogenases, which is supported by the finding that N2O did not inhibit corrinoid- independent fumarate-to-succinate reduction in strain SZ. Kinetic analyses revealed a best fit to the noncompetitive Michaelis-Menten inhibition model and determined N2O inhibitory constants, K-1, for PCE and cDCE dechlorination of 40.8 +/- 3.8 and 21.2 +/- 3.5 mu M in strain SZ and strain BAV1, respectively. The lowest K-1, value of 9.6 +/- 0.4 mu M was determined for VC to ethene reductive dechlorination in strain BAV1, suggesting that this crucial dechlorination step for achieving detoxification is most susceptible to N2O inhibition. Groundwater N2O concentrations exceeding 100 mu M are not uncommon, especially in watersheds impacted by nitrate runoff from agricultural sources. Thus, dissolved N2O measurements can inform about cDCE and VC stalls at sites impacted with chlorinated ethenes.
资助项目	Strategic Environmental Research and Development Program (SERDP project)[ER-2312] ; National Institute of Environmental Health Sciences[R01ES024294] ; National Science Foundation[DEB1831599] ; China Scholarship Council ; University of Tennessee
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000456349900015
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/123873]
专题	中国科学院沈阳应用生态研究所
通讯作者	Loffler, Frank E.
作者单位	1.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA 2.Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA 3.Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA 4.Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA 5.Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA 6.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Liaoning, Peoples R China 7.Bielefeld Univ, Fac Biol, RNA Biol & Mol Physiol, D-101133 Bielefeld, Germany
推荐引用方式 GB/T 7714	Yin, Yongchao,Yan, Jun,Chen, Gao,et al. Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(2):692-701.
APA	Yin, Yongchao,Yan, Jun,Chen, Gao,Murdoch, Fadime Kara,Pfisterer, Nina,&Loffler, Frank E..(2019).Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(2),692-701.
MLA	Yin, Yongchao,et al."Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.2(2019):692-701.