Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery

doi:10.1016/j.biortech.2016.01.126

	Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery
	Ma, Fang 1; Zhao, Feng 1,2; Zhou, Ji-Dong 2; Shi, Rong-Jiu 2; Han, Si-Qin 2; Zhang, Jie 1; Zhang, Ying 2
刊名	BIORESOURCE TECHNOLOGY
	2016-05-01
卷号	207 页码:24-30
关键词	Bio-competitive Exclusion Technology Engineered Strain Simulated Reservoir Conditions High-throughput Sequencing Microbial Communities
ISSN号	0960-8524
DOI	10.1016/j.biortech.2016.01.126
英文摘要	Sulfate-reducing bacteria (SRB) are widely existed in oil production system, and its H2S product inhibits rhamnolipid producing bacteria. In-situ production of rhamnolipid is promising for microbial enhanced oil recovery. Inhibition of SRB, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl were investigated. Strain Rhl can simultaneously remove S-2 (> 92%) and produce rhamnolipid (> 136 mg/l) under S-2 stress below 33.3 mg/l. Rhl reduced the SRB numbers from 109 to 105 cells/ml, and the production of H2S was delayed and decreased to below 2 mg/l. Rhl also produced rhamnolipid and removed S-2 under laboratory simulated oil reservoir conditions. High-throughput sequencing data demonstrated that addition of strain Rhl significantly changed the original microbial communities of oilfield production water and decreased the species and abundance of SRB. Bioaugmentation of strain Rhl in oilfield is promising for simultaneous control of SRB, removal of S-2 and enhance oil recovery. (C) 2016 Elsevier Ltd. All rights reserved.
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000371262600004
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122495]
专题	中国科学院沈阳应用生态研究所
通讯作者	Zhang, Ying
作者单位	1.Harbin Inst Technol, Sch Municipal & Environm Engn, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Liaoning Provin, Peoples R China
推荐引用方式 GB/T 7714	Ma, Fang,Zhao, Feng,Zhou, Ji-Dong,et al. Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery[J]. BIORESOURCE TECHNOLOGY,2016,207:24-30.
APA	Ma, Fang.,Zhao, Feng.,Zhou, Ji-Dong.,Shi, Rong-Jiu.,Han, Si-Qin.,...&Zhang, Ying.(2016).Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery.BIORESOURCE TECHNOLOGY,207,24-30.
MLA	Ma, Fang,et al."Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery".BIORESOURCE TECHNOLOGY 207(2016):24-30.