Superantigen staphylococcal enterotoxin C1 mutant can reduce paraquat pulmonary fibrosis

doi:10.3109/15376516.2015.1056863

	Superantigen staphylococcal enterotoxin C1 mutant can reduce paraquat pulmonary fibrosis
	Wang, Nana 3; Li, Tiegang 2; Xu, Mingkai 1; Zhao, Min 2
刊名	TOXICOLOGY MECHANISMS AND METHODS
	2015
卷号	25 期号:7 页码:574-580
关键词	Paraquat Pulmonary Fibrosis Staphylococcal Enterotoxin C1 Superantigen
ISSN号	1537-6516
DOI	10.3109/15376516.2015.1056863
英文摘要	A network of inflammation factors is related to pulmonary fibrosis induced by paraquat (PQ) poisoning. At high doses, the superantigen staphylococcal enterotoxin C1 (SEC1) can induce immunological unresponsiveness and inhibit release of inflammation factors. In this study, site-directed mutagenesis was performed at the H118 and H122 amino acid residues of SEC1 to reduce SEC1 toxicity. The SEC1 mutant showed significantly decreased pyrogenic toxicity, but retained clonal anergy at high dosages in vitro. Pretreatment with the SEC1 mutant prior to PQ poisoning in mice reduced symptom duration and severity, prolonged survival time, and decreased the splenocyte response to ConA induction. The SEC1 mutant also down-regulated several important cytokines related to fibrosis in the plasma after PQ poisoning. SEC1 decreased the expression of genes related to pulmonary fibrosis, including alpha-SMA, COL1a1, COL3 and TGF-beta 1, in PQ poisoned mice. Histomorphological observation indicated alleviation of pathological changes in the lungs after SEC1 pretreatment compared to mice in the PQ group. In conclusion, the SEC1 mutant reduced pulmonary interstitial fibrosis induced by PQ poisoning.
WOS研究方向	Toxicology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000369821700009
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122467]
专题	中国科学院沈阳应用生态研究所
通讯作者	Zhao, Min
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.China Med Univ, Shengjing Hosp, Emergency Dept, 36 Sanhao St, Shenyang 110004, Peoples R China 3.China Med Univ, Dept Endocrinol, Shengjing Hosp, Shenyang 110004, Peoples R China
推荐引用方式 GB/T 7714	Wang, Nana,Li, Tiegang,Xu, Mingkai,et al. Superantigen staphylococcal enterotoxin C1 mutant can reduce paraquat pulmonary fibrosis[J]. TOXICOLOGY MECHANISMS AND METHODS,2015,25(7):574-580.
APA	Wang, Nana,Li, Tiegang,Xu, Mingkai,&Zhao, Min.(2015).Superantigen staphylococcal enterotoxin C1 mutant can reduce paraquat pulmonary fibrosis.TOXICOLOGY MECHANISMS AND METHODS,25(7),574-580.
MLA	Wang, Nana,et al."Superantigen staphylococcal enterotoxin C1 mutant can reduce paraquat pulmonary fibrosis".TOXICOLOGY MECHANISMS AND METHODS 25.7(2015):574-580.