Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A

doi:10.1007/s12010-015-1832-7

	Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A
	Ma, Zongwang 1,2; Hu, Jiangchun 1
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2015-12-01
卷号	177 期号:7 页码:1520-1529
关键词	Bacillus Mojavensis Bacillus Surfactins Bioemulsifiers Chemical Identification Emulsification Activity
ISSN号	0273-2289
DOI	10.1007/s12010-015-1832-7
英文摘要	Bacillus mojavensis B0621A was isolated from the mantle of a pearl oyster Pinctada martensii collected from South China Sea. Semi-purified surfactins (225 mg L-1) were obtained by acid precipitation and vacuum flash chromatography. The component of the semi-purified surfactins was preliminarily analyzed by liquid chromatography mass spectrometer system, and the results showed that all these surfactins could be a group of homologues. Eight surfactin homologues were isolated and afforded by reversed phase high-performance liquid chromatography. Furthermore, their structure was characterized by mass spectrometry analysis combined with nuclear magnetic resonance spectroscopy techniques. These surfactins shared seven amino acids as peptide backbone and a saturated beta-hydroxy fatty acid chain residue (from C-13 to C-15), differed each other from peptide sequence in the position of Leu(7) or Val(7). All these surfactins had significant activity and stability of emulsification under various pH (from 7.0 to 12.0), temperature range (from 20 to 115 A degrees C) and sodium chloride concentration (from 2.5 to 20.0 %, w/v). Taken all together, these results indicated that B. mojavensis B0621A have potential to be an alternative source as a biological-derived emulsifying agent.
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	HUMANA PRESS INC
WOS记录号	WOS:000365165500011
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122285]
专题	中国科学院沈阳应用生态研究所
通讯作者	Hu, Jiangchun
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ma, Zongwang,Hu, Jiangchun. Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,177(7):1520-1529.
APA	Ma, Zongwang,&Hu, Jiangchun.(2015).Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,177(7),1520-1529.
MLA	Ma, Zongwang,et al."Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 177.7(2015):1520-1529.