Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure | |
Zeng, Yan1,2; Zhang, Dun1,2; Qi, Peng1,2; Zheng, Laibao1,2,3 | |
刊名 | SENSORS AND ACTUATORS B-CHEMICAL |
2018-07-15 | |
卷号 | 265页码:468-475 |
关键词 | Label free DNA junction G-quadruplex DNA DNA detection Lysozyme detection |
ISSN号 | 0925-4005 |
DOI | 10.1016/j.snb.2018.03.039 |
通讯作者 | Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn) |
英文摘要 | Label free analysis of multiplex analytes is of great demand in modern bio-analytical science. In this study, a novel DNA junction coupled with two different G-quadruplex DNA structures was demonstrated, achieving colorimetric and fluorescent signal report. Upon the introduction of different targets (including DNA and Lysozyme), the specific interaction between targets and sensing DNA junction could induce the reaction of exonuclease-stimulated recycling of target DNA and conformation transformation based on aptamer/lysozyme binding. With the integration of G-quadruplex/Hemin as colorimetric probe and G-quadruplex/PPIX as fluorescence probe, this label-free sensing system showed high sensitivity and selectivity for DNA and lysozyme detection. Furthermore, we also demonstrated that this novel sensing method could respond to these two targets independently and simultaneously, promising a great hope for its potential application in analytical fields. (C) 2018 Elsevier B.V. All rights reserved. |
资助项目 | National Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; China Postdoctoral Science Foundation[2016 M602199] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-ES02] |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE SA |
WOS记录号 | WOS:000430232500057 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/158938] |
专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
通讯作者 | Zhang, Dun; Qi, Peng |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,265:468-475. |
APA | Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2018).Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure.SENSORS AND ACTUATORS B-CHEMICAL,265,468-475. |
MLA | Zeng, Yan,et al."Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure".SENSORS AND ACTUATORS B-CHEMICAL 265(2018):468-475. |
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