Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method
Li, Qiuyu1,2,3,4; Zhao, Weihong1,2,3,4; Miao, Hui1,2,3; Han, Xiaotian1,2,3
刊名MARINE POLLUTION BULLETIN
2019-02-01
卷号139页码:136-140
关键词Marine phosphate Marine phosphate detection Phosphomolybdenum blue spectrophotometric method Reagents preservation
ISSN号0025-326X
DOI10.1016/j.marpolbul.2018.12.011
通讯作者Zhao, Weihong(whzhao@qdio.ac.cn)
英文摘要This work attempted to prolong the validity of the molybdate mixed solution and ascorbic acid solution used in the phosphomolybdenum blue spectrophotometric method by improving their preservation according to the influence factors. The results showed that the molybdate mixed solution can be directly preserved in darkness with validity over half a year. The ascorbic acid solution is influenced by light, temperature, pH, metal ions, oxygen, and bacteria. The validity of ascorbic acid is shortened as the temperature rises. Through keeping in darkness, adding complexing agents, adjusting pH, removing oxygen and sterilization, the validity of ascorbic acid solution was prolonged to over 2.7 times under 4 degrees C and over 5 times under 25 degrees C. At the same time, the hybrid solution of ascorbic acid solution and molybdate mixed solution should be preserved separately, otherwise the using effect is poor.
资助项目Open Financial Grant from Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0410] ; Joint Funds of the National Natural Science Foundation of China[U1606404] ; State Oceanic Administration, People's Republic of China
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种英语
出版者PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号WOS:000458227800017
内容类型期刊论文
源URL[http://ir.qdio.ac.cn/handle/337002/160843]  
专题海洋研究所_海洋生态与环境科学重点实验室
通讯作者Zhao, Weihong
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Li, Qiuyu,Zhao, Weihong,Miao, Hui,et al. Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method[J]. MARINE POLLUTION BULLETIN,2019,139:136-140.
APA Li, Qiuyu,Zhao, Weihong,Miao, Hui,&Han, Xiaotian.(2019).Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method.MARINE POLLUTION BULLETIN,139,136-140.
MLA Li, Qiuyu,et al."Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method".MARINE POLLUTION BULLETIN 139(2019):136-140.
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