Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?

doi:10.3389/fpls.2018.01545

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?
	Lin, Haiying 1; Sun, Tao 1; Zhou, Yi2 ; Gu, Ruiting 2; Zhang, Xiaomei2 ; Yang, Wei 1
刊名	FRONTIERS IN PLANT SCIENCE
	2018-10-24
卷号	9 页码:12
关键词	genes transcriptomic analysis heavy metals seagrass Zostera japonica
ISSN号	1664-462X
DOI	10.3389/fpls.2018.01545
英文摘要	Healthy seagrasses are considered a prime indicator of estuarine and coastal ecosystem function; however, as the only group of flowering plants recolonizing the sea, seagrasses are frequently exposed to anthropogenic heavy metal pollutants, which are associated with high levels of molecular damage. To determine whether biologically relevant concentrations of heavy metals cause systematic alterations in RNA expression patterns, we performed a gene expression study using transcriptome analyses (RNA-seq). We exposed the typical intertidal seagrass Zostera japonica to 0 and 50 mu M of copper (Cu), lead (Pb), and cadmium (Cd) under laboratory conditions. A total of 18,266 differentially expressed genes (DEGs) were identified, of which 2001 co-expressed genes directly related by Cu, Pb, and Cd stress. We also examined the effects of short-term heavy metal Cu, Pb, and Cd pulses on the accumulation of metals in Z. japonica and showed metal concentrations were higher in the shoots than in roots. Twelve differentially expressed genes were further analyzed for expression differences using real-time quantitative polymerase chain reaction (RT-qPCR). Our data suggest that as coastal seawater pollution worsens, the sensitive genes identified in this study may be useful biomarkers of sublethal effects and provide fundamental information for Z. japonica resistant gene engineering.
资助项目	National Science & Technology Basic Work Program[2015FY110600] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; key Research and Development Project of Shandong Province[2017GHY15111] ; National Marine Public Welfare Research Project[201305043] ; National Key Research and Development Program of China[2018YFC1406404] ; Key Research Project of Frontier Science of Chinese Academy of Sciences[QYZDB-SSWDQC041-1] ; National Natural Science Foundation of China[41176140]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000448111500001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/160446]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Sun, Tao; Zhou, Yi
作者单位	1.Beijing Normal Univ, State Key Lab Water Environm Simulat, Sch Environm, Beijing, Peoples R China 2.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Lin, Haiying,Sun, Tao,Zhou, Yi,et al. Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?[J]. FRONTIERS IN PLANT SCIENCE,2018,9:12.
APA	Lin, Haiying,Sun, Tao,Zhou, Yi,Gu, Ruiting,Zhang, Xiaomei,&Yang, Wei.(2018).Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?.FRONTIERS IN PLANT SCIENCE,9,12.
MLA	Lin, Haiying,et al."Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?".FRONTIERS IN PLANT SCIENCE 9(2018):12.