Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)

doi:10.1016/j.cbpb.2018.08.002

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)
	Xue, Rui 1,2,3,4; Wang, Xueying1,2,4 ; Xu, Shihong 1,2,4; Liu, Yifan 1,2,4; Feng, Chengcheng1,2,4 ; Zhao, Chunyan1,2,4 ; Liu, Qinghua 1,2,4; Li, Jun 1,2,4
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2018-12-01
卷号	226 页码:53-63
关键词	Autosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein
ISSN号	1096-4959
DOI	10.1016/j.cbpb.2018.08.002
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
英文摘要	Egg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire process of egg yolk generation in female turbot (Scophthalmus maximus), we obtained full-length cDNAs of vtg genes, examined gene expression in the female liver and ovary, and analyzed Vtg synthesis in the ovary. Three distinct complete polypeptide sequences were identified and designated as VtgAa, VtgAb, and VtgC, which confirmed the multiplicity of the vtg gene in turbot and showed that it follows a "three vtg model". The expression of these three vtg genes in the female liver was far higher than that in other tissues, including the ovary. The expression of all three vtg genes was extremely low before vitellogenesis, and then increased and was maintained at a high level until the degradation stage, which was in accordance with changes in the concentration of estradiol-17 beta (E-2) and the gonadosomatic index. Compared with the liver, the ovary had a higher E-2 level and lower vtg expression, suggesting that some other factors limit high vtg expression in the ovary of turbot. Transcripts of vtgAb and the Yps derived from them were both detected in oogonia and primary oocytes, which showed that these might possess the ability to perform autosynthesis of yolk. These findings add to our understanding of the reproductive physiology of Vtg synthesis in turbot.
资助项目	National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS (2014) ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; improved Seeds Engineering Project of Shandong Province (2014-2017)
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000447114400007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/160460]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Xue, Rui,Wang, Xueying,Xu, Shihong,et al. Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,226:53-63.
APA	Xue, Rui.,Wang, Xueying.,Xu, Shihong.,Liu, Yifan.,Feng, Chengcheng.,...&Li, Jun.(2018).Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,226,53-63.
MLA	Xue, Rui,et al."Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 226(2018):53-63.