A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells

doi:10.1016/j.carbpol.2018.08.096

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells
	Geng, Lihua 1,2,3,4; Hu, Weicheng 5; Liu, Yingjuan 1,2,3; Wang, Jing1,2,3,4 ; Zhang, Quanbin 1,2,3,4
刊名	CARBOHYDRATE POLYMERS
	2018-12-01
卷号	201 页码:557-565
关键词	Saccharina japonica Polysaccharide Immunomodulatory activity Macrophage
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2018.08.096
通讯作者	Zhang, Quanbin(qbzhang@qdio.ac.cn)
英文摘要	A heteropolysaccharide (SHP) with a strong immunomodulatory effect on RAW 264.7 cells was prepared from Saccharina japonica. Chemical analysis demonstrated that SHP was primarily composed of mannose, glucuronic acid, glucose, fucose, galactose, xylose and rahmnose with a molar ratio of 1.00:0.85:0.84:0.58:0.30:0.37:0.15. ESI-MS showed that depolymerized SHP produced oligo-glucuronan, oligo-glucuronomannan, sulfated fuco-oligosaccharides and other hetero-oligosaccharides. The in vitro immunomodulatory results showed that SHP could increase NO production and up-regulate the expression of many immune effectors, including iNOS, COX-2 and TNF-alpha, displaying an apparent immune enhancement activities. Western blot analysis proved that SHP activated the expression levels of many key components involved in NF-kappa B, MAPK and Akt signaling pathways. Our results together indicated that SHP has the potential to be developed as a novel immunomodulator for activating immune system.
资助项目	Fujian STS project of Chinese Academy of Sciences ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Key Research and Development Project of Shandong Province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190] ; K.C. Wong Education Foundation, CAS
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000445030100060
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/160229]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Quanbin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Huaian 223300, Peoples R China
推荐引用方式 GB/T 7714	Geng, Lihua,Hu, Weicheng,Liu, Yingjuan,et al. A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells[J]. CARBOHYDRATE POLYMERS,2018,201:557-565.
APA	Geng, Lihua,Hu, Weicheng,Liu, Yingjuan,Wang, Jing,&Zhang, Quanbin.(2018).A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells.CARBOHYDRATE POLYMERS,201,557-565.
MLA	Geng, Lihua,et al."A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells".CARBOHYDRATE POLYMERS 201(2018):557-565.