Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia

doi:10.1016/j.aquaculture.2018.02.021

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia
	Han, Si-yin1,2,3 ; Wang, Meng-qiang1,2 ; Liu, Mei 1,2; Wang, Bao-jie 1,2; Jiang, Ke-yong 1,2; Wang, Lei 1,2
刊名	AQUACULTURE
	2018-03-01
卷号	490 页码:44-52
关键词	Litopenaeus vannamei Hepatopancreas Midgut Cyclic serious/medium hypoxia Oxidative stress Gene expression
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2018.02.021
通讯作者	Wang, Lei(wanglei@qdio.ac.cn)
英文摘要	Low dissolved oxygen (DO) could cause a stress response in shrimp, the objective of this study was to compare sensitivity of the hepatopancreas and midgut to oxidative stress under low DO. The white shrimp Litopenaeus vannamei were reared under conditions of cyclic serious/medium hypoxia (CSMH, 0.8-3.5 mg/L) versus normoxia (N, 6.4-7.5 mg/L) for a 28-day period. Survival and growth performance of shrimp were evaluated, and hypoxia inducible factors 1a (HIF-1 alpha) expression, antioxidant responses, apoptosis and histology in the hepatopancreas and midgut were investigated. Antioxidant responses tested included the following: manganese superoxide dismutase, glutathione peroxidases, glutathione-S-transferase, metallothionein and heat shock protein70 expression. Results showed enhanced HIF-1 alpha expression and antioxidant responses in the hepatopancreas and midgut during short-term cyclic serious/medium hypoxia (<= 7 days), which suggested early adaptive mechanism of shrimp to tolerate low DO and avoid oxidative damage. Meanwhile, HIF-1 alpha expression, apoptosis and histopathological lesions were induced earlier in the hepatopancreas than the midgut. Thus, the hepatopancreas could be more sensitive to low DO and its oxidative stress than the midgut. However, long-term (>= 14 days) cyclic serious/medium hypoxia could disrupt cellular antioxidant mechanism with depressed antioxidant responses, and then aggravate apoptosis and histopathological lesions in the hepatopancreas and midgut, particularly the hepatopancreas would lose antioxidant ability earlier than the midgut because of higher sensitivity to oxidative stress. Therefore, we have a few insights that it is vital to protect hepatopancreas for controlling shrimp death and growth inhibition under cyclic serious/medium hypoxia.
资助项目	STS Key Deployment Project of Chinese Academy of Sciences[KFZD-SW-106]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000429323600007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158802]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Han, Si-yin,Wang, Meng-qiang,Liu, Mei,et al. Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia[J]. AQUACULTURE,2018,490:44-52.
APA	Han, Si-yin,Wang, Meng-qiang,Liu, Mei,Wang, Bao-jie,Jiang, Ke-yong,&Wang, Lei.(2018).Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia.AQUACULTURE,490,44-52.
MLA	Han, Si-yin,et al."Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia".AQUACULTURE 490(2018):44-52.