Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera

doi:10.7717/peerj.4373

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera
	Wang, Xueqin ; Yu, Huahua; Xing, Ronge; Chen, Xiaolin ; Liu, Song ; Li, Pengcheng1
刊名	PEERJ
	2018-02-15
卷号	6 页码:21
关键词	Mackerel viscera Protease Response surface methodology Antioxidant activity
ISSN号	2167-8359
DOI	10.7717/peerj.4373
通讯作者	Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要	Mackerel (Pneumatophorus japonicus) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 degrees C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various in vitro assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.
资助项目	National Natural Science Foundation of China[41506175] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Qingdao Science and Technology Project[17-3-3-21-nsh] ; Chinese Academy of Sciences[KFZD-SW-106] ; Science and Technology Development Plan Project of Shandong Province[2016YYSP010]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PEERJ INC
WOS记录号	WOS:000425362200006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158072]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera[J]. PEERJ,2018,6:21.
APA	Wang, Xueqin,Yu, Huahua,Xing, Ronge,Chen, Xiaolin,Liu, Song,&Li, Pengcheng.(2018).Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera.PEERJ,6,21.
MLA	Wang, Xueqin,et al."Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera".PEERJ 6(2018):21.